2rmk
From Proteopedia
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|PDB= 2rmk |SIZE=350|CAPTION= <scene name='initialview01'>2rmk</scene> | |PDB= 2rmk |SIZE=350|CAPTION= <scene name='initialview01'>2rmk</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Protein_kinase_C Protein kinase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.13 2.7.11.13] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_kinase_C Protein kinase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.13 2.7.11.13] </span> |
|GENE= RAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PKN1, PKN, PRK1, PRKCL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= RAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PKN1, PKN, PRK1, PRKCL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rmk OCA], [http://www.ebi.ac.uk/pdbsum/2rmk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rmk RCSB]</span> | ||
}} | }} | ||
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[[Category: Nietlispach, D.]] | [[Category: Nietlispach, D.]] | ||
[[Category: Owen, D.]] | [[Category: Owen, D.]] | ||
| - | [[Category: GCP]] | ||
| - | [[Category: MG]] | ||
[[Category: adp-ribosylation]] | [[Category: adp-ribosylation]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:02:07 2008'' |
Revision as of 02:02, 31 March 2008
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| Ligands: | , | ||||||
| Gene: | RAC1 (Homo sapiens), PKN1, PKN, PRK1, PRKCL1 (Homo sapiens) | ||||||
| Activity: | Protein kinase C, with EC number 2.7.11.13 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Rac1/PRK1 Complex
Overview
Protein kinase C-related kinase 1 (PRK1 or PKN) is involved in regulation of the intermediate filaments of the actin cytoskeleton, as well as having effects on processes as diverse as mitotic timing and apoptosis. It is activated by interacting with the Rho family small G proteins and arachidonic acid or by caspase cleavage. We have previously shown that the HR1b of PRK1 binds exclusively to Rac1, whereas the HR1a domain binds to both Rac1 and RhoA. Here, we have determined the solution structure of the HR1b-Rac complex. We show that HR1b binds to the C-terminal end of the effector loop and switch 2 of Rac1. Comparison with the HR1a-RhoA structure shows that this part of the Rac1-HR1b interaction is homologous to one of the contact sites that HR1a makes with RhoA. The Rac1 used in this study included the C-terminal polybasic region, which is frequently omitted from structural studies, as well as the core G domain. The Rac1 C-terminal region reverses in direction to interact with residues in switch 2, and the polybasic region itself interacts with residues in HR1b. The interactions with HR1b do not prevent the polybasic region being available to contact the negatively charged membrane phospholipids, which is considered to be its primary role. This is the first structural demonstration that the C terminus of a G protein forms a novel recognition element for effector binding.
About this Structure
2RMK is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The Rac1 polybasic region is required for interaction with its effector PRK1., Modha R, Campbell LJ, Nietlispach D, Buhecha HR, Owen D, Mott HR, J Biol Chem. 2008 Jan 18;283(3):1492-500. Epub 2007 Nov 15. PMID:18006505
Page seeded by OCA on Mon Mar 31 05:02:07 2008
Categories: Homo sapiens | Protein complex | Protein kinase C | Buhecha, H R. | Campbell, L J. | Modha, R. | Mott, H R. | Nietlispach, D. | Owen, D. | Adp-ribosylation | Alternative splicing | Atp-binding | Cytoplasm | Effector | G protein | Gtp-binding | Kinase | Lipoprotein | Membrane | Membrane protein/transferase complex | Methylation | Nucleotide-binding | Phosphorylation | Polymorphism | Prenylation | Serine/threonine-protein kinase | Transferase
