6g8b
From Proteopedia
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<StructureSection load='6g8b' size='340' side='right' caption='[[6g8b]], [[Resolution|resolution]] 2.37Å' scene=''> | <StructureSection load='6g8b' size='340' side='right' caption='[[6g8b]], [[Resolution|resolution]] 2.37Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6g8b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G8B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6g8b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G8B FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7MF:[(7~{S})-1-BROMANYL-6,6-BIS(OXIDANYL)-4-PHENYL-5,7,8,9-TETRAHYDROBENZO[7]ANNULEN-7-YL]AZANIUM'>7MF</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7MF:[(7~{S})-1-BROMANYL-6,6-BIS(OXIDANYL)-4-PHENYL-5,7,8,9-TETRAHYDROBENZO[7]ANNULEN-7-YL]AZANIUM'>7MF</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pepN, b0932, JW0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Membrane_alanyl_aminopeptidase Membrane alanyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.2 3.4.11.2] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Membrane_alanyl_aminopeptidase Membrane alanyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.2 3.4.11.2] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g8b OCA], [http://pdbe.org/6g8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g8b RCSB], [http://www.ebi.ac.uk/pdbsum/6g8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g8b ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g8b OCA], [http://pdbe.org/6g8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g8b RCSB], [http://www.ebi.ac.uk/pdbsum/6g8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g8b ProSAT]</span></td></tr> | ||
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/AMPN_ECOLI AMPN_ECOLI]] Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation. | [[http://www.uniprot.org/uniprot/AMPN_ECOLI AMPN_ECOLI]] Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The accuracy of X-ray diffraction data is directly related to how the X-ray detector records photons. Here we describe the application of a direct-detection charge-integrating pixel-array detector (JUNGFRAU) in macromolecular crystallography (MX). JUNGFRAU features a uniform response on the subpixel level, linear behavior toward high photon rates, and low-noise performance across the whole dynamic range. We demonstrate that these features allow accurate MX data to be recorded at unprecedented speed. We also demonstrate improvements over previous-generation detectors in terms of data quality, using native single-wavelength anomalous diffraction (SAD) phasing, for thaumatin, lysozyme, and aminopeptidase N. Our results suggest that the JUNGFRAU detector will substantially improve the performance of synchrotron MX beamlines and equip them for future synchrotron light sources. | ||
| + | |||
| + | Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector.,Leonarski F, Redford S, Mozzanica A, Lopez-Cuenca C, Panepucci E, Nass K, Ozerov D, Vera L, Olieric V, Buntschu D, Schneider R, Tinti G, Froejdh E, Diederichs K, Bunk O, Schmitt B, Wang M Nat Methods. 2018 Oct;15(10):799-804. doi: 10.1038/s41592-018-0143-7. Epub 2018, Oct 1. PMID:30275593<ref>PMID:30275593</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6g8b" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Ecoli]] | ||
[[Category: Membrane alanyl aminopeptidase]] | [[Category: Membrane alanyl aminopeptidase]] | ||
[[Category: Leonarski, F]] | [[Category: Leonarski, F]] | ||
Revision as of 06:59, 24 October 2018
E. coli Aminopeptidase N solved by Native SAD from a dataset collected in 60 second with JUNGFRAU detector
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