2mni

Publication Abstract from PubMed

Q4D059 (UniProt accession number), is an 86-residue protein from Trypanosoma cruzi, conserved in the related kinetoplastid parasites Trypanosoma brucei and Leishmania major. These pathogens are the causal agents of the neglected diseases: Chagas, sleeping sickness and leishmaniases respectively and had recently their genomes sequenced. Q4D059 shows low sequence similarity with mammal proteins and because of its essentiality demonstrated in T. brucei, it is a potential target for anti-parasitic drugs. The 11 hypothetical proteins homologous to Q4D059 are all uncharacterized proteins of unknown function. Here, the solution structure of Q4D059 was solved by NMR and its backbone dynamics was characterized by 15N relaxation parameters. The structure is composed by a parallel/anti-parallel three-stranded beta-sheet packed against four helical regions. The structure is well defined by ca. 9 NOEs per residue and a backbone rmsd of 0.50+/-0.05A for the representative ensemble of 20 lowest-energy structures. The structure is overall rigid except for N-terminal residues A9 to D11 at the beginning of beta1, K38, V39 at the end of helix H3 with rapid motion in the ps-ns timescale and G25 (helix H2), I68 (beta2) and V78 (loop 3) undergoing internal motion in the mus-ms timescale. Limited structural similarities were found in protein structures deposited in the PDB, therefore functional inferences based on protein structure information are not clear. Q4D059 adopts a alpha/beta fold that is slightly similar to the ATPase sub-domain IIB of the heat-shock protein 70 (HSP70) and to the N-terminal domain of the ribosomal protein L11.

NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi.,Lopez-Castilla A, Pons T, Pires JR J Struct Biol. 2015 Mar 5. pii: S1047-8477(15)00053-2. doi:, 10.1016/j.jsb.2015.02.007. PMID:25748338^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.