6ews
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution Structure of Rhabdopeptide NRPS Docking Domain Kj12A-NDD== | |
| + | <StructureSection load='6ews' size='340' side='right' caption='[[6ews]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ews]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EWS FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ews FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ews OCA], [http://pdbe.org/6ews PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ews RCSB], [http://www.ebi.ac.uk/pdbsum/6ews PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ews ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Several peptides in clinical use are derived from non-ribosomal peptide synthetases (NRPS). In these systems multiple NRPS subunits interact with each other in a specific linear order mediated by specific docking domains (DDs), whose structures are not known yet, to synthesize well-defined peptide products. In contrast to classical NRPSs, single-module NRPS subunits responsible for the generation of rhabdopeptide/xenortide-like peptides (RXPs) can act in different order depending on subunit stoichiometry thereby producing peptide libraries. To define the basis for their unusual interaction patterns, we determine the structures of all N-terminal DDs ((N)DDs) as well as of an (N)DD-(C)DD complex and characterize all putative DD interactions thermodynamically for such a system. Key amino acid residues for DD interactions are identified that upon their exchange change the DD affinity and result in predictable changes in peptide production. Recognition rules for DD interactions are identified that also operate in other megasynthase complexes. | ||
| - | + | Structure-based redesign of docking domain interactions modulates the product spectrum of a rhabdopeptide-synthesizing NRPS.,Hacker C, Cai X, Kegler C, Zhao L, Weickhmann AK, Wurm JP, Bode HB, Wohnert J Nat Commun. 2018 Oct 19;9(1):4366. doi: 10.1038/s41467-018-06712-1. PMID:30341296<ref>PMID:30341296</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6ews" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Bode, H B]] | ||
[[Category: Cai, X]] | [[Category: Cai, X]] | ||
| + | [[Category: Hacker, C]] | ||
| + | [[Category: Kegler, C]] | ||
| + | [[Category: Weickhmann, A K]] | ||
[[Category: Woehnert, J]] | [[Category: Woehnert, J]] | ||
| - | [[Category: | + | [[Category: Zhao, L]] |
| + | [[Category: Peptide binding protein]] | ||
| + | [[Category: Protein]] | ||
Revision as of 06:55, 31 October 2018
Solution Structure of Rhabdopeptide NRPS Docking Domain Kj12A-NDD
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