3bwd

From Proteopedia

(Difference between revisions)

Revision as of 07:31, 31 October 2018

Crystal structure of the plant Rho protein ROP5

Structural highlights

3bwd is a 1 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2ntx, 2nty
Gene:	ARAC6, RAC2, ROP5 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RAC6_ARATH] May be involved in cell polarity control during the actin-dependent tip growth of pollen tubes.^[1] ^[2] Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation (By similarity).^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The small G protein ROP5 from the model plant Arabidopsis thaliana was purified and crystallized using the hanging-drop vapour-diffusion method. ROP5 crystals were obtained using PEG 3000 as precipitant and belong to space group P2(1). A data set was collected to 1.53 A resolution using synchrotron radiation at 100 K. A clear molecular-replacement solution was found using ROP4-GDP of the ROP4-GDP-PRONE8 complex as the search model.

Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5.,Thomas C, Berken A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1070-2. Epub 2007 Nov 30. PMID:18084097^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH. Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth. J Cell Biol. 1999 Apr 19;145(2):317-30. PMID:10209027
↑ Li H, Wu G, Ware D, Davis KR, Yang Z. Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast. Plant Physiol. 1998 Oct;118(2):407-17. PMID:9765526
↑ Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH. Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth. J Cell Biol. 1999 Apr 19;145(2):317-30. PMID:10209027
↑ Li H, Wu G, Ware D, Davis KR, Yang Z. Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast. Plant Physiol. 1998 Oct;118(2):407-17. PMID:9765526
↑ Thomas C, Berken A. Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1070-2. Epub 2007 Nov 30. PMID:18084097 doi:10.1107/S1744309107059672

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bwd"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the plant Rho protein ROP5==
 <StructureSection load='3bwd' size='340' side='right' caption='[[3bwd]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ntx|2ntx]], [[2nty|2nty]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARAC6, RAC2, ROP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwd OCA], [http://pdbe.org/3bwd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bwd RCSB], [http://www.ebi.ac.uk/pdbsum/3bwd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwd OCA], [http://pdbe.org/3bwd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bwd RCSB], [http://www.ebi.ac.uk/pdbsum/3bwd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bwd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bwd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bwd_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 39: / Line 40: @@
 [[Category: Berken, A]]
 [[Category: Thomas, C]]
+[[Category: Cytoplasm]]
 [[Category: G domain]]
 [[Category: Gtp-binding]]

3bwd

From Proteopedia

Revision as of 07:31, 31 October 2018

Crystal structure of the plant Rho protein ROP5

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox