2by9
From Proteopedia
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[[Category: synchrotron radiation]] | [[Category: synchrotron radiation]] | ||
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Revision as of 15:47, 5 November 2007
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IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION
Overview
This paper focuses on the radiation-damage effects when applying the same, total X-ray dose to protein crystals at different dose rates. These, experiments have been performed on both a selenomethionated protein and on, bovine trypsin using dose rates that span nearly two orders of magnitude., The results show no clear dose-rate effect on the global indicators of, radiation damage, but a small measurable dose-rate effect could be found, when studying specific radiation damage. It is hypothesized that this, observed dose-rate effect relates to differences in the steady-state, free-radical concentration.
About this Structure
2BY9 is a Single protein structure of sequence from Bos taurus with SO4, CA, BAM and GOL as ligands. Active as Trypsin, with EC number 3.4.21.4 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442
Page seeded by OCA on Mon Nov 5 17:52:47 2007
Categories: Bos taurus | Single protein | Trypsin | Leiros, H.K.S. | Mcsweeney, S.M. | Ravelli, R.B.G. | Timmins, J. | BAM | CA | GOL | SO4 | Data collection | Dose-rate | Hydrolase | Radiation damage | Synchrotron radiation
