6g12
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of GMPPNP bound RbgA from S. aureus== | |
| + | <StructureSection load='6g12' size='340' side='right' caption='[[6g12]], [[Resolution|resolution]] 1.93Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6g12]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G12 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G12 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g12 OCA], [http://pdbe.org/6g12 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g12 RCSB], [http://www.ebi.ac.uk/pdbsum/6g12 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g12 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/A0A0H2XK72_STAA3 A0A0H2XK72_STAA3]] Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity.[PIRNR:PIRNR006230] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Efficient adaptation to environmental changes is pivotal for all bacterial cells. Almost all bacterial species depend on the conserved stringent response system to prompt timely transcriptional and metabolic responses according to stress conditions and nutrient depletion. The stringent response relies on the stress-dependent synthesis of the second messenger nucleotides and alarmones (p)ppGpp, which pleiotropically target and reprogram processes that consume cellular resources, such as ribosome biogenesis. Here we show that (p)ppGpp acts on the ribosome biogenesis GTPase A (RbgA) of Gram-positive bacteria. Using X-ray crystallography, hydrogen-deuterium exchange mass spectrometry (HDX-MS) and kinetic analysis, we demonstrate that the alarmones (p)ppGpp bind to RbgA in a manner similar to that of binding by GDP and GTP and therby act as competitive inhibitors. Our structural analysis of Staphylococcus aureus RbgA bound to ppGpp and pppGpp at 1.8 A and 1.65 A resolution, respectively, suggested that the alarmones (p)ppGpp prevent the active GTPase conformation of RbgA by sterically blocking the association of its G2 motif via their 3'-pyrophosphate moieties. Taken together, our structural and biochemical characterization of RbgA in the context of the alarmone-mediated stringent response reveals how (p)ppGpp affects the function of RbgA and reprograms this GTPase to arrest the ribosomal large subunit. | ||
| - | + | Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.,Pausch P, Steinchen W, Wieland M, Klaus T, Freibert SA, Altegoer F, Wilson DN, Bange G J Biol Chem. 2018 Oct 26. pii: RA118.003070. doi: 10.1074/jbc.RA118.003070. PMID:30366986<ref>PMID:30366986</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 6g12" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bange, G]] | [[Category: Bange, G]] | ||
[[Category: Pausch, P]] | [[Category: Pausch, P]] | ||
| + | [[Category: Cpgtpase]] | ||
| + | [[Category: Rna binding protein]] | ||
Revision as of 12:17, 7 November 2018
Crystal structure of GMPPNP bound RbgA from S. aureus
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