6ci2
From Proteopedia
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<StructureSection load='6ci2' size='340' side='right' caption='[[6ci2]], [[Resolution|resolution]] 1.96Å' scene=''> | <StructureSection load='6ci2' size='340' side='right' caption='[[6ci2]], [[Resolution|resolution]] 1.96Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6ci2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ci2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anoxybacillus_kamchatkensis_g10 Anoxybacillus kamchatkensis g10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI2 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ci2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci2 OCA], [http://pdbe.org/6ci2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ci2 RCSB], [http://www.ebi.ac.uk/pdbsum/6ci2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci2 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ci2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci2 OCA], [http://pdbe.org/6ci2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ci2 RCSB], [http://www.ebi.ac.uk/pdbsum/6ci2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nonribosomal peptide synthetases (NRPSs) increase the chemical diversity of their products by acquiring tailoring domains. Linear gramicidin synthetase starts with a tailoring formylation (F) domain, which likely originated from a sugar formyltransferase (FT) gene. Here, we present studies on an Anoxybacillus kamchatkensis sugar FT representative of the prehorizontal gene transfer FT. Gene cluster analysis reveals that this FT acts on a UDP-sugar in a novel pathway for synthesis of a 7-formamido derivative of CMP-pseudaminic acid. We recapitulate the pathway up to and including the formylation step in vitro, experimentally demonstrating the role of the FT. We also present X-ray crystal structures of the FT alone and with ligands, which unveil contrasts with other structurally characterized sugar FTs and show close structural similarity with the F domain. The structures reveal insights into the adaptations that were needed to co-opt and evolve a sugar FT into a functional and useful NRPS domain. | ||
| + | |||
| + | Structural Insight into a Novel Formyltransferase and Evolution to a Nonribosomal Peptide Synthetase Tailoring Domain.,Reimer JM, Harb I, Ovchinnikova OG, Jiang J, Whitfield C, Schmeing TM ACS Chem Biol. 2018 Oct 30. doi: 10.1021/acschembio.8b00739. PMID:30346688<ref>PMID:30346688</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ci2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Anoxybacillus kamchatkensis g10]] | ||
[[Category: Harb, I]] | [[Category: Harb, I]] | ||
[[Category: Jiang, J]] | [[Category: Jiang, J]] | ||
Revision as of 12:40, 7 November 2018
Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis
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