6hos
From Proteopedia
(Difference between revisions)
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<StructureSection load='6hos' size='340' side='right' caption='[[6hos]], [[Resolution|resolution]] 2.15Å' scene=''> | <StructureSection load='6hos' size='340' side='right' caption='[[6hos]], [[Resolution|resolution]] 2.15Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6hos]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HOS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hos]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_28485 Atcc 28485]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HOS FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATY40_BA7504148 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4922 ATCC 28485])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hos OCA], [http://pdbe.org/6hos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hos RCSB], [http://www.ebi.ac.uk/pdbsum/6hos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hos ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hos OCA], [http://pdbe.org/6hos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hos RCSB], [http://www.ebi.ac.uk/pdbsum/6hos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hos ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cell-cell and cell-substrate based adhesion of yeasts are major determinants of their adoption of different life styles. Genome-mining of ascomycetous GPI-anchored cell wall proteins with lectin-like PA14 domains identified a unique class of putative adhesins in the clade of methylotrophic Komagataella yeasts, many of which are known to colonize plants and insects involving yet unknown adhesion mechanisms. Here, we report the functional and structural analysis of two of its members: KpFlo1 (=Cea1), that is highly specific for terminal N-acetylglucosamine moieties, and KpFlo2, which represents an orphan lectin with intact binding site but unknown specificity. Crystal structures of the Cea1 adhesion domain complexed to N-acetylglucosamine and N,N'-diacetylchitobiose reveal a Ca(2+)-dependent binding mode that differs from other members of the PA14/Flo5 adhesin family. Heterologous expression of Cea1A in Saccharomyces cerevisiae promotes cellular adhesion to non-reducing ends of non-crystalline chitin. Overall, our data suggest that high-affinity recognition of beta-GlcNAc-capped glycans by Cea1 enable Komagataella species to interact with surface cues present in fungi and insects. | ||
| + | |||
| + | Structural and Functional Characterization of PA14/Flo5-Like Adhesins From Komagataella pastoris.,Kock M, Bruckner S, Wozniak N, Maestre-Reyna M, Veelders M, Schlereth J, Mosch HU, Essen LO Front Microbiol. 2018 Oct 30;9:2581. doi: 10.3389/fmicb.2018.02581. eCollection, 2018. PMID:30425696<ref>PMID:30425696</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6hos" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 28485]] | ||
[[Category: Essen, L O]] | [[Category: Essen, L O]] | ||
[[Category: Kock, M]] | [[Category: Kock, M]] | ||
Revision as of 21:05, 2 December 2018
Structure of the KpFlo2 adhesin domain in complex with glycerol
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