2zbf
From Proteopedia
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|PDB= 2zbf |SIZE=350|CAPTION= <scene name='initialview01'>2zbf</scene>, resolution 2.40Å | |PDB= 2zbf |SIZE=350|CAPTION= <scene name='initialview01'>2zbf</scene>, resolution 2.40Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TG1:OCTANOIC+ACID+[3S-[3ALPHA,+3ABETA,+4ALPHA,+6BETA,+6ABETA,+7BETA,+8ALPHA(Z),+9BALPHA]]-6-(ACETYLOXY)-2,3,-3A,4,5,6,6A,7,8,9B-DECAHYDRO-3,3A-DIHYDROXY-3,6,9-TRIMETHYL-8-[(2-METHYL-1-OXO-2-BUTENYL)OXY]-2-OXO-4-(1-OXOBUTOXY)-AZULENO[4,5-B]FURAN-7-YL+ESTER'>TG1</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1su4|1SU4]], [[1iwo|1IWO]], [[1vfp|1VFP]], [[2zbd|2ZBD]], [[1wpg|1WPG]], [[2agv|2AGV]], [[2dqs|2DQS]], [[2ear|2EAR]], [[2eas|2EAS]], [[2eat|2EAT]], [[2eau|2EAU]], [[2zbe|2ZBE]], [[2zbg|2ZBG]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbf OCA], [http://www.ebi.ac.uk/pdbsum/2zbf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zbf RCSB]</span> | ||
}} | }} | ||
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[[Category: Ogawa, H.]] | [[Category: Ogawa, H.]] | ||
[[Category: Toyoshima, C.]] | [[Category: Toyoshima, C.]] | ||
| - | [[Category: ACE]] | ||
| - | [[Category: BEF]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: TG1]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
| Line 48: | Line 47: | ||
[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:08 2008'' |
Revision as of 02:20, 31 March 2008
| |||||||
| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Activity: | Calcium-transporting ATPase, with EC number 3.6.3.8 | ||||||
| Related: | 1SU4, 1IWO, 1VFP, 2ZBD, 1WPG, 2AGV, 2DQS, 2EAR, 2EAS, 2EAT, 2EAU, 2ZBE, 2ZBG
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Calcium pump crystal structure with bound BeF3 and TG in the absence of calcium
Overview
Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum is the best-studied member of the P-type or E1/E2 type ion transporting ATPases. It has been crystallized in seven different states that cover nearly the entire reaction cycle. Here we describe the structure of this ATPase complexed with phosphate analogs BeF(3)(-) and AlF(4)(-) in the absence of Ca(2+), which correspond to the E2P ground state and E2 approximately P transition state, respectively. The luminal gate is open with BeF(3)(-) and closed with AlF(4)(-). These and the E1 approximately P.ADP analog crystal structures show that a two-step rotation of the cytoplasmic A-domain opens and closes the luminal gate through the movements of the M1-M4 transmembrane helices. There are several conformational switches coupled to the rotation, and the one in the cytoplasmic part of M2 has critical importance. In the second step of rotation, positioning of one water molecule couples the hydrolysis of aspartylphosphate to closing of the gate.
About this Structure
2ZBF is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump., Toyoshima C, Norimatsu Y, Iwasawa S, Tsuda T, Ogawa H, Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19831-6. Epub 2007 Dec 5. PMID:18077416
Page seeded by OCA on Mon Mar 31 05:20:08 2008
Categories: Calcium-transporting ATPase | Oryctolagus cuniculus | Single protein | Norimatsu, Y. | Ogawa, H. | Toyoshima, C. | Alternative splicing | Atp-binding | Calcium | Calcium transport | Endoplasmic reticulum | Had fold | Hydrolase | Ion transport | Magnesium | Membrane protein | Metal-binding | Nucleotide-binding | P-type atpase | Phosphorylation | Sarcoplasmic reticulum | Transmembrane | Transport
