2zex
From Proteopedia
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|PDB= 2zex |SIZE=350|CAPTION= <scene name='initialview01'>2zex</scene>, resolution 1.20Å | |PDB= 2zex |SIZE=350|CAPTION= <scene name='initialview01'>2zex</scene>, resolution 1.20Å | ||
|SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Residue+B+301'>AC1</scene>, <scene name='pdbsite=AC2:Bgc+Binding+Site+For+Residue+B+302'>AC2</scene>, <scene name='pdbsite=AC3:Bgc+Binding+Site+For+Residue+B+303'>AC3</scene>, <scene name='pdbsite=AC4:Bgc+Binding+Site+For+Residue+B+304'>AC4</scene>, <scene name='pdbsite=AC5:Bgc+Binding+Site+For+Residue+B+305'>AC5</scene>, <scene name='pdbsite=AC6:Bgc+Binding+Site+For+Residue+A+401'>AC6</scene>, <scene name='pdbsite=AC7:Bgc+Binding+Site+For+Residue+A+402'>AC7</scene>, <scene name='pdbsite=AC8:Bgc+Binding+Site+For+Residue+A+403'>AC8</scene>, <scene name='pdbsite=AC9:Bgc+Binding+Site+For+Residue+A+404'>AC9</scene>, <scene name='pdbsite=BC1:Bgc+Binding+Site+For+Residue+A+405'>BC1</scene>, <scene name='pdbsite=BC2:Ca+Binding+Site+For+Residue+B+306'>BC2</scene> and <scene name='pdbsite=BC3:Ca+Binding+Site+For+Residue+A+406'>BC3</scene> | |SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Residue+B+301'>AC1</scene>, <scene name='pdbsite=AC2:Bgc+Binding+Site+For+Residue+B+302'>AC2</scene>, <scene name='pdbsite=AC3:Bgc+Binding+Site+For+Residue+B+303'>AC3</scene>, <scene name='pdbsite=AC4:Bgc+Binding+Site+For+Residue+B+304'>AC4</scene>, <scene name='pdbsite=AC5:Bgc+Binding+Site+For+Residue+B+305'>AC5</scene>, <scene name='pdbsite=AC6:Bgc+Binding+Site+For+Residue+A+401'>AC6</scene>, <scene name='pdbsite=AC7:Bgc+Binding+Site+For+Residue+A+402'>AC7</scene>, <scene name='pdbsite=AC8:Bgc+Binding+Site+For+Residue+A+403'>AC8</scene>, <scene name='pdbsite=AC9:Bgc+Binding+Site+For+Residue+A+404'>AC9</scene>, <scene name='pdbsite=BC1:Bgc+Binding+Site+For+Residue+A+405'>BC1</scene>, <scene name='pdbsite=BC2:Ca+Binding+Site+For+Residue+B+306'>BC2</scene> and <scene name='pdbsite=BC3:Ca+Binding+Site+For+Residue+A+406'>BC3</scene> | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= celA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= celA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=500 Thermomicrobium roseum]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2zew|2ZEW]], [[2zey|2ZEY]], [[2zez|2ZEZ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zex OCA], [http://www.ebi.ac.uk/pdbsum/2zex PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zex RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ZEX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2ZEX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEX OCA]. |
==Reference== | ==Reference== | ||
Molecular basis for the selectivity and specificity of ligand recognition by the family 16 carbohydrate binding modules from thermoanaerobacterium polysaccharolyticum manA., Bae B, Ohene-Adjei S, Kocherginskaya S, Mackie RI, Spies MA, Cann IK, Nair SK, J Biol Chem. 2007 Nov 29;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18025086 18025086] | Molecular basis for the selectivity and specificity of ligand recognition by the family 16 carbohydrate binding modules from thermoanaerobacterium polysaccharolyticum manA., Bae B, Ohene-Adjei S, Kocherginskaya S, Mackie RI, Spies MA, Cann IK, Nair SK, J Biol Chem. 2007 Nov 29;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18025086 18025086] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: | + | [[Category: Thermomicrobium roseum]] |
[[Category: Bae, B.]] | [[Category: Bae, B.]] | ||
[[Category: Nair, S K.]] | [[Category: Nair, S K.]] | ||
| - | [[Category: CA]] | ||
[[Category: family 16 cbm-1 cellopentaose complex]] | [[Category: family 16 cbm-1 cellopentaose complex]] | ||
[[Category: glycosidase]] | [[Category: glycosidase]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:40 2008'' |
Revision as of 02:20, 31 March 2008
| |||||||
| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , and | ||||||
| Ligands: | , | ||||||
| Gene: | celA (Thermomicrobium roseum) | ||||||
| Related: | 2ZEW, 2ZEY, 2ZEZ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Family 16 carbohydrate binding module
Overview
Enzymes that hydrolyze complex polysaccharides into simple sugars are modular in architecture and consist of single or multiple catalytic domains fused to targeting modules called carbohydrate binding modules (CBMs). CBMs bind to their ligands with high affinity, and increase the efficiency of the catalytic components by targeting the enzymes to its substrate. Here, we utilized a multi-disciplinary approach to characterize each of the two family 16 carbohydrate binding domain component of the highly active mannanase from the thermophile T. polysaccharolyticum. These represent the first crystal structures of family 16 CBMs. Calorimetric analysis show that while these CBMs demonstrate high specificity towards ss-1,4 linked sugars, they can engage both cello- and manno-polysaccharides. In order to elucidate the molecular basis for this specificity and selectivity, we have determined high resolution crystal structures of each of the two carbohydrate binding modules, as well as of binary complexes of CBM16-1 bound to either mannopentaose or cellopentaose. These results provide detailed molecular insights into ligand recognition and yield a framework for rational engineering experiments designed to expand the natural repertoire of these targeting modules.
About this Structure
2ZEX is a Single protein structure of sequence from Thermomicrobium roseum. Full crystallographic information is available from OCA.
Reference
Molecular basis for the selectivity and specificity of ligand recognition by the family 16 carbohydrate binding modules from thermoanaerobacterium polysaccharolyticum manA., Bae B, Ohene-Adjei S, Kocherginskaya S, Mackie RI, Spies MA, Cann IK, Nair SK, J Biol Chem. 2007 Nov 29;. PMID:18025086
Page seeded by OCA on Mon Mar 31 05:20:40 2008
