2zfd
From Proteopedia
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|PDB= 2zfd |SIZE=350|CAPTION= <scene name='initialview01'>2zfd</scene>, resolution 1.20Å | |PDB= 2zfd |SIZE=350|CAPTION= <scene name='initialview01'>2zfd</scene>, resolution 1.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | |GENE= CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1uhn|1UHN]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB]</span> | ||
}} | }} | ||
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[[Category: Sato, M.]] | [[Category: Sato, M.]] | ||
[[Category: Shimizu, T.]] | [[Category: Shimizu, T.]] | ||
| - | [[Category: ACY]] | ||
| - | [[Category: CA]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: calcium binding protein]] | [[Category: calcium binding protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:45 2008'' |
Revision as of 02:20, 31 March 2008
| |||||||
| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | CBL2 (Arabidopsis thaliana), CIPK14 (Arabidopsis thaliana) | ||||||
| Related: | 1UHN
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14
Overview
Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B-like (CBL) proteins and their target kinases, CBL-interacting protein kinases (CIPKs), represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL motif) within the C-terminal regulatory domain. To better understand the functional role of the CBL-CIPK system, we determined the crystal structure of AtCBL2 in complex with the regulatory domain of AtCIPK14 at 1.2 A resolution. The NAF/FISL motif is inserted into a hydrophobic crevice within AtCBL2, accompanied by a large displacement of the helices and loop on the opposite side of the NAF/FISL motif from the C-terminal region, which shields the hydrophobic crevice in free form. Ca(2+) are coordinated within four EF hands in AtCBL2 in bound form. This calcium coordination pattern differs from that in the structure of the SOS3-SOS2 complex previously reported. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.
About this Structure
2ZFD is a Protein complex structure of sequences from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14., Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T, J Mol Biol. 2008 Jan 11;. PMID:18237745
Page seeded by OCA on Mon Mar 31 05:20:45 2008
Categories: Arabidopsis thaliana | Protein complex | Akaboshi, M. | Hashimoto, H. | Ishida, H. | Koizumi, N. | Sato, M. | Shimizu, T. | Atp-binding | Calcium binding protein | Kinase | Nucleotide-binding | Protein-protein complex | Serine/threonine-protein kinase | Signaling protein/transferase complex | Transferase
