377d

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|PDB= 377d |SIZE=350|CAPTION= <scene name='initialview01'>377d</scene>, resolution 1.760&Aring;
|PDB= 377d |SIZE=350|CAPTION= <scene name='initialview01'>377d</scene>, resolution 1.760&Aring;
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=377d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=377d OCA], [http://www.ebi.ac.uk/pdbsum/377d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=377d RCSB]</span>
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:55 2008''

Revision as of 02:21, 31 March 2008


PDB ID 377d

Drag the structure with the mouse to rotate
, resolution 1.760Å
Ligands: , , ,
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



5'-R(*CP*GP*UP*AP*CP*G)-3'


Overview

The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes.

About this Structure

377D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

1.76 A structure of a pyrimidine start alternating A-RNA hexamer r(CGUAC)dG., Biswas R, Mitra SN, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):570-6. PMID:9761851

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