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3fi1

From Proteopedia

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Revision as of 08:00, 5 December 2018

NhaA dimer model

Structural highlights

3fi1 is a 1 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NHAA_ECOLI] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

NhaA, the main sodium-proton exchanger in the inner membrane of Escherichia coli, regulates the cytosolic concentrations of H and Na. It is inactive at acidic pH, becomes active between pH 6 and pH 7, and reaches maximum activity at pH 8. By cryo-electron microscopy of two-dimensional crystals grown at pH 4 and incubated at higher pH, we identified two sequential conformational changes in the protein in response to pH or substrate ions. The first change is induced by a rise in pH from 6 to 7 and marks the transition from the inactive state to the pH-activated state. pH activation, which precedes the ion-induced conformational change, is accompanied by an overall expansion of the NhaA monomer and a local ordering of the N-terminus. The second conformational change is induced by the substrate ions Na and Li at pH above 7 and involves a 7-A displacement of helix IVp. This movement would cause a charge imbalance at the ion-binding site that may trigger the release of the substrate ion and open a periplasmic exit channel.

Conformations of NhaA, the Na/H exchanger from Escherichia coli, in the pH-activated and ion-translocating states.,Appel M, Hizlan D, Vinothkumar KR, Ziegler C, Kuhlbrandt W J Mol Biol. 2009 Feb 20;386(2):351-65. Epub 2008 Dec 25. PMID:19135453^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Taglicht D, Padan E, Schuldiner S. Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. J Biol Chem. 1991 Jun 15;266(17):11289-94. PMID:1645730
↑ Appel M, Hizlan D, Vinothkumar KR, Ziegler C, Kuhlbrandt W. Conformations of NhaA, the Na/H exchanger from Escherichia coli, in the pH-activated and ion-translocating states. J Mol Biol. 2009 Feb 20;386(2):351-65. Epub 2008 Dec 25. PMID:19135453 doi:10.1016/j.jmb.2008.12.042

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fi1"

@@ Line 1: / Line 1: @@
 ==NhaA dimer model==
 <StructureSection load='3fi1' size='340' side='right' caption='[[3fi1]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3fi1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FI1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3fi1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FI1 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fi1 OCA], [http://pdbe.org/3fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/3fi1 PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fi1 OCA], [http://pdbe.org/3fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/3fi1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fi1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 11: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fi1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fi1_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 30: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli k-12]]
 [[Category: Appel, M]]
 [[Category: Hizlan, D]]

3fi1

From Proteopedia

Revision as of 08:00, 5 December 2018

NhaA dimer model

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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