Arnold lab: coenzyme specificity
From Proteopedia
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The Arnold lab wanted to establish a generalized procedure to change the cofactor specificity of certain enzymes from NADPH to NADH. <ref>PMID:23776225</ref> | The Arnold lab wanted to establish a generalized procedure to change the cofactor specificity of certain enzymes from NADPH to NADH. <ref>PMID:23776225</ref> | ||
| - | <StructureSection load=' | + | <StructureSection load='' size='340' side='right' caption='NADPH cofactor binding to KARI enzyme (4kmw)' scene=' |
| + | The KARI enzyme from ''S. exigua'' binds to NADPH. To change the cofactor specificity of the <scene name='80/802749/Nadph_binding_site/1''> | ||
==Active site of the enzyme== | ==Active site of the enzyme== | ||
| - | The KARI enzyme from ''S. exigua'' binds to NADPH. To change the cofactor specificity of the <scene name='80/802749/Nadph_binding_site/1'>binding | + | The KARI enzyme from ''S. exigua'' binds to NADPH. To change the cofactor specificity of the <scene name='80/802749/Nadph_binding_site/1'>binding site</scene>, the researchers took a structure-based directed evolution approach. Try to guess which residues they might have targeted for mutagenesis based on the structure (which was determined after successfully changing the specificity of the enzyme). |
Revision as of 22:35, 6 December 2018
The Arnold lab wanted to establish a generalized procedure to change the cofactor specificity of certain enzymes from NADPH to NADH. [1]
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References
- ↑ Brinkmann-Chen S, Flock T, Cahn JK, Snow CD, Brustad EM, McIntosh JA, Meinhold P, Zhang L, Arnold FH. General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH. Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):10946-51. doi:, 10.1073/pnas.1306073110. Epub 2013 Jun 17. PMID:23776225 doi:10.1073/pnas.1306073110
