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3gn4

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Revision as of 05:16, 7 December 2018

Myosin lever arm

Structural highlights

3gn4 is a 6 chain structure with sequence from Drome and Pig. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	MYO6 (PIG), Cam, CG8472 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MYO6_PIG] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).^[1] [CALM_DROME] Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Myosin VI challenges the prevailing theory of how myosin motors move on actin: the lever arm hypothesis. While the reverse directionality and large powerstroke of myosin VI can be attributed to unusual properties of a subdomain of the motor (converter with a unique insert), these adaptations cannot account for the large step size on actin. Either the lever arm hypothesis needs modification, or myosin VI has some unique form of extension of its lever arm. We determined the structure of the region immediately distal to the lever arm of the motor and show that it is a three-helix bundle. Based on C-terminal truncations that display the normal range of step sizes on actin, CD, fluorescence studies, and a partial deletion of the bundle, we demonstrate that this bundle unfolds upon dimerization of two myosin VI monomers. This unconventional mechanism generates an extension of the lever arm of myosin VI.

Myosin VI dimerization triggers an unfolding of a three-helix bundle in order to extend its reach.,Mukherjea M, Llinas P, Kim H, Travaglia M, Safer D, Menetrey J, Franzini-Armstrong C, Selvin PR, Houdusse A, Sweeney HL Mol Cell. 2009 Aug 14;35(3):305-15. Epub 2009 Aug 6. PMID:19664948^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Naccache SN, Hasson T. Myosin VI altered at threonine 406 stabilizes actin filaments in vivo. Cell Motil Cytoskeleton. 2006 Oct;63(10):633-45. PMID:16917816 doi:http://dx.doi.org/10.1002/cm.20150
↑ Mukherjea M, Llinas P, Kim H, Travaglia M, Safer D, Menetrey J, Franzini-Armstrong C, Selvin PR, Houdusse A, Sweeney HL. Myosin VI dimerization triggers an unfolding of a three-helix bundle in order to extend its reach. Mol Cell. 2009 Aug 14;35(3):305-15. Epub 2009 Aug 6. PMID:19664948 doi:10.1016/j.molcel.2009.07.010

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gn4"

@@ Line 1: / Line 1: @@
 ==Myosin lever arm==
 <StructureSection load='3gn4' size='340' side='right' caption='[[3gn4]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MYO6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG]), Cam, CG8472 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gn4 OCA], [http://pdbe.org/3gn4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gn4 RCSB], [http://www.ebi.ac.uk/pdbsum/3gn4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gn4 OCA], [http://pdbe.org/3gn4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gn4 RCSB], [http://www.ebi.ac.uk/pdbsum/3gn4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gn4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/3gn4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/3gn4_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 50: / Line 51: @@
 [[Category: Zong, A B]]
 [[Category: 3-helix bundle]]
+[[Category: Acetylation]]
 [[Category: Actin-binding]]
 [[Category: Atp-binding]]
+[[Category: Calcium]]
 [[Category: Calmodulin-binding]]
+[[Category: Coiled coil]]
+[[Category: Cytoplasm]]
 [[Category: Endocytosis]]
 [[Category: Golgi apparatus]]

3gn4

From Proteopedia

Revision as of 05:16, 7 December 2018

Myosin lever arm

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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