3b4a
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 3b4a |SIZE=350|CAPTION= <scene name='initialview01'>3b4a</scene>, resolution 2.7Å | |PDB= 3b4a |SIZE=350|CAPTION= <scene name='initialview01'>3b4a</scene>, resolution 2.7Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=A23:ADENOSINE-5'-PHOSPHATE-2',3'-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2z74|2z74]], [[2z75|2z75]], [[2ho7|2ho7]], [[3b4b|3B4B]], [[3b4c|3B4C]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4a OCA], [http://www.ebi.ac.uk/pdbsum/3b4a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b4a RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Amare, A R.Ferre-D.]] | [[Category: Amare, A R.Ferre-D.]] | ||
[[Category: Klein, D J.]] | [[Category: Klein, D J.]] | ||
| - | [[Category: GLP]] | ||
| - | [[Category: MG]] | ||
[[Category: glucosamine-6-phosphate]] | [[Category: glucosamine-6-phosphate]] | ||
[[Category: riboswitch]] | [[Category: riboswitch]] | ||
| Line 31: | Line 32: | ||
[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:55 2008'' |
Revision as of 02:22, 31 March 2008
| |||||||
| , resolution 2.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Related: | 2z74, 2z75, 2ho7, 3B4B, 3B4C
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate
Overview
The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
About this Structure
3B4A is a Protein complex structure of sequences from Thermoanaerobacter tengcongensis. Full crystallographic information is available from OCA.
Reference
Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:17990888
Page seeded by OCA on Mon Mar 31 05:22:55 2008
