3bgw
From Proteopedia
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= G40P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= G40P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10724 Bacillus phage SPP1]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[3bho|3BHO]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bgw OCA], [http://www.ebi.ac.uk/pdbsum/3bgw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bgw RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3BGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 3BGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spp1 Bacillus phage spp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGW OCA]. |
==Reference== | ==Reference== | ||
The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18157148 18157148] | The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18157148 18157148] | ||
| - | [[Category: | + | [[Category: Bacillus phage spp1]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, X S.]] | [[Category: Chen, X S.]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:26:07 2008'' |
Revision as of 02:26, 31 March 2008
| |||||||
| , resolution 3.91Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | G40P (Bacillus phage SPP1) | ||||||
| Related: | 3BHO
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase
Overview
Helicases are essential enzymes for DNA replication, a fundamental process in all living organisms. The DnaB family are hexameric replicative helicases that unwind duplex DNA and coordinate with RNA primase and other proteins at the replication fork in prokaryotes. Here, we report the full-length crystal structure of G40P, a DnaB family helicase. The hexamer complex reveals an unusual architectural feature and a new type of assembly mechanism. The hexamer has two tiers: a three-fold symmetric N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with two different conformations, termed cis and trans, come together to provide a topological solution for the dual symmetry within a hexamer. Structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. This study provides insights into the structural and functional interplay between G40P helicase and DnaG primase.
About this Structure
3BGW is a Single protein structure of sequence from Bacillus phage spp1. Full crystallographic information is available from OCA.
Reference
The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:18157148
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