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3bjz

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|PDB= 3bjz |SIZE=350|CAPTION= <scene name='initialview01'>3bjz</scene>, resolution 2.40&Aring;
|PDB= 3bjz |SIZE=350|CAPTION= <scene name='initialview01'>3bjz</scene>, resolution 2.40&Aring;
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+198'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+198'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+C+198'>AC3</scene> and <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+D+198'>AC4</scene>
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+198'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+198'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+C+198'>AC3</scene> and <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+D+198'>AC4</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
+
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= gmhA, PA4425 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])
|GENE= gmhA, PA4425 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])
 +
|DOMAIN=
 +
|RELATEDENTRY=[[1x92|1X92]], [[2i22|2I22]], [[2i2w|2I2W]]
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjz OCA], [http://www.ebi.ac.uk/pdbsum/3bjz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bjz RCSB]</span>
}}
}}
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[[Category: Savchenko, A.]]
[[Category: Savchenko, A.]]
[[Category: Walker, J R.]]
[[Category: Walker, J R.]]
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[[Category: CL]]
 
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[[Category: SO4]]
 
[[Category: alpha/beta protein]]
[[Category: alpha/beta protein]]
[[Category: carbohydrate metabolism]]
[[Category: carbohydrate metabolism]]
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[[Category: sis domain]]
[[Category: sis domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:59:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:03 2008''

Revision as of 02:27, 31 March 2008

Image:3bjz.jpg


PDB ID 3bjz

Drag the structure with the mouse to rotate
, resolution 2.40Å
Sites: , , and
Ligands: , ,
Gene: gmhA, PA4425 (Pseudomonas aeruginosa PAO1)
Related: 1X92, 2I22, 2I2W


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Pseudomonas aeruginosa phosphoheptose isomerase


Overview

The barrier imposed by lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria presents a significant challenge in treatment of these organisms with otherwise effective hydrophobic antibiotics. The absence of l-glycero-d-manno-heptose in the LPS molecule is associated with a dramatically increased bacterial susceptibility to hydrophobic antibiotics and thus enzymes in the ADP-heptose biosynthesis pathway are of significant interest. GmhA catalyzes the isomerization of d-sedoheptulose 7-phosphate into d-glycero-d-manno-heptose 7-phosphate, the first committed step in the formation of ADP-heptose. Here we report structures of GmhA from Escherichia coli and Pseudomonas aeruginosa in apo, substrate, and product-bound forms, which together suggest that GmhA adopts two distinct conformations during isomerization through reorganization of quaternary structure. Biochemical characterization of GmhA mutants, combined with in vivo analysis of LPS biosynthesis and novobiocin susceptibility, identifies key catalytic residues. We postulate GmhA acts through an enediol-intermediate isomerase mechanism.

About this Structure

3BJZ is a Single protein structure of sequence from Pseudomonas aeruginosa pao1. Full crystallographic information is available from OCA.

Reference

Structure and Function of Sedoheptulose-7-phosphate Isomerase, a Critical Enzyme for Lipopolysaccharide Biosynthesis and a Target for Antibiotic Adjuvants., Taylor PL, Blakely KM, de Leon GP, Walker JR, McArthur F, Evdokimova E, Zhang K, Valvano MA, Wright GD, Junop MS, J Biol Chem. 2008 Feb 1;283(5):2835-45. Epub 2007 Dec 3. PMID:18056714

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