6dhb
From Proteopedia
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<StructureSection load='6dhb' size='340' side='right' caption='[[6dhb]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='6dhb' size='340' side='right' caption='[[6dhb]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6dhb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHB FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HAVCR2, TIM3, TIMD3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhb OCA], [http://pdbe.org/6dhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhb RCSB], [http://www.ebi.ac.uk/pdbsum/6dhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhb ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhb OCA], [http://pdbe.org/6dhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhb RCSB], [http://www.ebi.ac.uk/pdbsum/6dhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/HAVR2_HUMAN HAVR2_HUMAN]] Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.<ref>PMID:14556005</ref> <ref>PMID:16286920</ref> | [[http://www.uniprot.org/uniprot/HAVR2_HUMAN HAVR2_HUMAN]] Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.<ref>PMID:14556005</ref> <ref>PMID:16286920</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | T-cell immunoglobulin and mucin domain containing protein-3 (TIM-3) is an important immune regulator. Here, we describe a novel high resolution (1.7 A) crystal structure of the human (h)TIM-3 N-terminal variable immunoglobulin (IgV) domain with bound calcium (Ca(++)) that was confirmed by nuclear magnetic resonance (NMR) spectroscopy. Significant conformational differences were observed in the B-C, C'-C'' and C'-D loops of hTIM-3 compared to mouse (m)TIM-3, hTIM-1 and hTIM-4. Further, the conformation of the C-C' loop of hTIM-3 was notably different from hTIM-4. Consistent with the known metal ion-dependent binding of phosphatidylserine (PtdSer) to mTIM-3 and mTIM-4, the NMR spectral analysis and crystal structure of Ca(++)-bound hTIM-3 revealed that residues in the hTIM-3 F-G loop coordinate binding to Ca(++). In addition, we established a novel biochemical assay to define hTIM-3 functionality as determined by binding to human carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). These studies provide new insights useful for understanding and targeting hTIM-3. | ||
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| + | High resolution X-ray and NMR structural study of human T-cell immunoglobulin and mucin domain containing protein-3.,Gandhi AK, Kim WM, Sun ZJ, Huang YH, Bonsor DA, Sundberg EJ, Kondo Y, Wagner G, Kuchroo VK, Petsko G, Blumberg RS Sci Rep. 2018 Nov 30;8(1):17512. doi: 10.1038/s41598-018-35754-0. PMID:30504845<ref>PMID:30504845</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6dhb" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Blumberg, R S]] | [[Category: Blumberg, R S]] | ||
[[Category: Bonsor, D]] | [[Category: Bonsor, D]] | ||
Revision as of 08:51, 19 December 2018
Crystal structure of the human TIM-3 with bound Calcium
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