3hts
From Proteopedia
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|PDB= 3hts |SIZE=350|CAPTION= <scene name='initialview01'>3hts</scene>, resolution 1.75Å | |PDB= 3hts |SIZE=350|CAPTION= <scene name='initialview01'>3hts</scene>, resolution 1.75Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hts OCA], [http://www.ebi.ac.uk/pdbsum/3hts PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3hts RCSB]</span> | ||
}} | }} | ||
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[[Category: Littlefield, O.]] | [[Category: Littlefield, O.]] | ||
[[Category: Nelson, H C.M.]] | [[Category: Nelson, H C.M.]] | ||
| - | [[Category: GOL]] | ||
[[Category: complex (winged helix_turn_ helix/dna)]] | [[Category: complex (winged helix_turn_ helix/dna)]] | ||
[[Category: dna-binding protein]] | [[Category: dna-binding protein]] | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:33:56 2008'' |
Revision as of 02:33, 31 March 2008
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| , resolution 1.75Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX
Overview
The 1.75 A crystal structure of the Kluyveromyces lactis heat shock transcription factor (HSF) DNA-binding domain (DBD) complexed with DNA reveals a protein-DNA interface with few direct major groove contacts and a number of phosphate backbone contacts that are primarily water-mediated interactions. The DBD, a 'winged' helix-turn-helix protein, displays a novel mode of binding in that the 'wing' does not contact DNA like all others of that class. Instead, the monomeric DBD, which crystallized as a symmetric dimer to a pair of nGAAn inverted repeats, uses the 'wing' to form part of the protein-protein contacts. This dimer interface is likely important for increasing the DNA-binding specificity and affinity of the trimeric form of HSF, as well as for increasing cooperativity between adjacent trimers.
About this Structure
3HTS is a Single protein structure of sequence from Kluyveromyces lactis. Full crystallographic information is available from OCA.
Reference
A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal., Littlefield O, Nelson HC, Nat Struct Biol. 1999 May;6(5):464-70. PMID:10331875
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