5z6p
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of an agarase, AgWH50C== |
| + | <StructureSection load='5z6p' size='340' side='right' caption='[[5z6p]], [[Resolution|resolution]] 2.06Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5z6p]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6P FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6p OCA], [http://pdbe.org/5z6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z6p RCSB], [http://www.ebi.ac.uk/pdbsum/5z6p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | AgWH50C, an exo-beta-agarase of GH50 isolated from Agarivorans gilvus WH0801, plays a key role in the enzymatic production of neoagarobiose, which has great application prospect in the cosmetics and pharmaceutical industry. In contrast, the poor thermostability becomes the main obstructive factor of glycoside hydrolase (GH) family 50 agarases, including AgWH50C. Herein, based on the AgWH50C crystal structure, we designed several mutants by a multiple cross-linked rational design protocol used thermostability predicting softwares ETSS, PoPMuSiC, and HotMuSiC. To our surprise, the mutant K621F increased its relative activity by as much as 45% and the optimal temperature increased to 38 degrees C compared to that of wild-type, AgWH50C (30 degrees C). The thermostability of K621F also exhibited a substantial improvement. Considering that the gelling temperature of the agarose is higher than 35 degrees C, K621F can be used to hydrolyze agarose for neoagarobiose production. | ||
| - | + | Structure-based design of agarase AgWH50C from Agarivorans gilvus WH0801 to enhance thermostability.,Zhang P, Zhang J, Zhang L, Sun J, Li Y, Wu L, Zhou J, Xue C, Mao X Appl Microbiol Biotechnol. 2018 Dec 6. pii: 10.1007/s00253-018-9540-1. doi:, 10.1007/s00253-018-9540-1. PMID:30523371<ref>PMID:30523371</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5z6p" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Li, Y]] | [[Category: Li, Y]] | ||
| - | [[Category: Zhang, J | + | [[Category: Mao, X]] |
| - | [[Category: Zhang, P | + | [[Category: Zhang, J]] |
| + | [[Category: Zhang, L]] | ||
| + | [[Category: Zhang, P]] | ||
| + | [[Category: Zhou, J]] | ||
| + | [[Category: Agarase activity]] | ||
| + | [[Category: Glucoside hydrolase 50 family]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 06:07, 2 January 2019
The crystal structure of an agarase, AgWH50C
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