6mua
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Csm1-Csm4 subcomplex in the type III-A CRISPR-Csm interference complex== | |
| + | <StructureSection load='6mua' size='340' side='right' caption='[[6mua]], [[Resolution|resolution]] 2.91Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6mua]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUA FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mua OCA], [http://pdbe.org/6mua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mua RCSB], [http://www.ebi.ac.uk/pdbsum/6mua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mua ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type IotaIotaIota CRISPR-Cas systems provide robust immunity against foreign RNA and DNA by sequence-specific RNase and target RNA-activated sequence-nonspecific DNase and RNase activities. We report on cryo-EM structures of Thermococcus onnurineus Csm(crRNA) binary, Csm(crRNA)-target RNA and Csm(crRNA)-target RNA(anti-tag) ternary complexes in the 3.1 A range. The topological features of the crRNA 5'-repeat tag explains the 5'-ruler mechanism for defining target cleavage sites, with accessibility of positions -2 to -5 within the 5'-repeat serving as sensors for avoidance of autoimmunity. The Csm3 thumb elements introduce periodic kinks in the crRNA-target RNA duplex, facilitating cleavage of the target RNA with 6-nt periodicity. Key Glu residues within a Csm1 loop segment of Csm(crRNA) adopt a proposed autoinhibitory conformation suggestive of DNase activity regulation. These structural findings, complemented by mutational studies of key intermolecular contacts, provide insights into Csm(crRNA) complex assembly, mechanisms underlying RNA targeting and site-specific periodic cleavage, regulation of DNase cleavage activity, and autoimmunity suppression. | ||
| - | + | Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity.,Jia N, Mo CY, Wang C, Eng ET, Marraffini LA, Patel DJ Mol Cell. 2018 Nov 27. pii: S1097-2765(18)30977-8. doi:, 10.1016/j.molcel.2018.11.007. PMID:30503773<ref>PMID:30503773</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6mua" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Jia, N]] | ||
| + | [[Category: Patel, D J]] | ||
| + | [[Category: Csm1-csm4 subcomplex]] | ||
| + | [[Category: Immune system]] | ||
| + | [[Category: Type iii-a crispr-cas system]] | ||
Revision as of 06:41, 9 January 2019
Crystal structure of Csm1-Csm4 subcomplex in the type III-A CRISPR-Csm interference complex
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