471d
From Proteopedia
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|PDB= 471d |SIZE=350|CAPTION= <scene name='initialview01'>471d</scene>, resolution 2.70Å | |PDB= 471d |SIZE=350|CAPTION= <scene name='initialview01'>471d</scene>, resolution 2.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=A44:2'-O-METHYOXYETHYL-ADENOSINE+5'-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2'-O-METHYOXYETHYL-CYTIDINE-5'-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2'-O-METHYOXYETHYL-GUANOSINE-5'-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U36:2'-O-METHYOXYETHYL-URIDINE-5'-MONOPHOSPHATE'>U36</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=471d OCA], [http://www.ebi.ac.uk/pdbsum/471d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=471d RCSB]</span> | ||
}} | }} | ||
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[[Category: Teplova, M.]] | [[Category: Teplova, M.]] | ||
[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
| - | [[Category: MG]] | ||
[[Category: 2'-o-(2-methoxyethyl) ribose]] | [[Category: 2'-o-(2-methoxyethyl) ribose]] | ||
[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
[[Category: rna dodecamer duplex]] | [[Category: rna dodecamer duplex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:37:38 2008'' |
Revision as of 02:37, 31 March 2008
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| , resolution 2.70Å | |||||||
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| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA
Overview
2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.
About this Structure
471D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355
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