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3mjx

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Revision as of 13:32, 16 January 2019

Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin

Structural highlights

3mjx is a 1 chain structure with sequence from Dicdi and Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	2jhr, 2jj9, 3bz7, 3bz8, 3bz9, 1yv3
Gene:	mhcA, DDB_G0286355 (DICDI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Publication Abstract from PubMed

We have identified pentabromopseudilin (PBP) as a potent inhibitor of myosin-dependent processes such as isometric tension development and unloaded shortening velocity. PBP-induced reductions in the rate constants for ATP binding, ATP hydrolysis and ADP dissociation extend the time required per myosin ATPase cycle in the absence and presence of actin. Additionally, coupling between the actin and nucleotide binding sites is reduced in the presence of the inhibitor. The selectivity of PBP differs from that observed with other myosin inhibitors. To elucidate the binding mode of PBP, we crystallized the Dictyostelium myosin-2 motor domain in the presence of Mg(2+)-ADP-meta-vanadate and PBP. The electron density for PBP is unambiguous and shows PBP to bind at a previously unknown allosteric site near the tip of the 50-kDa domain, at a distance of 16 A from the nucleotide binding site and 7.5 A away from the blebbistatin binding pocket.

The mechanism of pentabromopseudilin inhibition of myosin motor activity.,Fedorov R, Bohl M, Tsiavaliaris G, Hartmann FK, Taft MH, Baruch P, Brenner B, Martin R, Knolker HJ, Gutzeit HO, Manstein DJ Nat Struct Mol Biol. 2009 Jan;16(1):80-8. Epub 2009 Jan 4. PMID:19122661^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fedorov R, Bohl M, Tsiavaliaris G, Hartmann FK, Taft MH, Baruch P, Brenner B, Martin R, Knolker HJ, Gutzeit HO, Manstein DJ. The mechanism of pentabromopseudilin inhibition of myosin motor activity. Nat Struct Mol Biol. 2009 Jan;16(1):80-8. Epub 2009 Jan 4. PMID:19122661 doi:10.1038/nsmb.1542

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3mjx"

@@ Line 3: / Line 3: @@
 <StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735] and [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi] and [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jhr|2jhr]], [[2jj9|2jj9]], [[3bz7|3bz7]], [[3bz8|3bz8]], [[3bz9|3bz9]], [[1yv3|1yv3]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://pdbe.org/3mjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>
 </table>
@@ Line 27: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 11735]]
+[[Category: Dicdi]]
 [[Category: Dictyostelium discoideum]]
 [[Category: Baruch, P]]

3mjx

From Proteopedia

Revision as of 13:32, 16 January 2019

Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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