This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7rsa
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 7rsa |SIZE=350|CAPTION= <scene name='initialview01'>7rsa</scene>, resolution 1.26Å | |PDB= 7rsa |SIZE=350|CAPTION= <scene name='initialview01'>7rsa</scene>, resolution 1.26Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7rsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsa OCA], [http://www.ebi.ac.uk/pdbsum/7rsa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7rsa RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Gilliland, G L.]] | [[Category: Gilliland, G L.]] | ||
[[Category: Wlodawer, A.]] | [[Category: Wlodawer, A.]] | ||
| - | [[Category: DOD]] | ||
| - | [[Category: TBU]] | ||
[[Category: hydrolase (phosphoric diester)]] | [[Category: hydrolase (phosphoric diester)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:44:44 2008'' |
Revision as of 02:44, 31 March 2008
| |||||||
| , resolution 1.26Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS
Overview
The structure of phosphate-free bovine ribonuclease A has been refined at 1.26-A resolution by a restrained least-squares procedure to a final R factor of 0.15. X-ray diffraction data were collected with an electronic position-sensitive detector. The final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen side chains were modeled with two alternate conformations. Major changes to the active site include the addition of two waters in the phosphate-binding pocket, disordering of Gln-11, and tilting of the imidazole ring of His-119. The structure of the protein and of the associated solvent was extensively compared with three other high-resolution, refined structures of this enzyme.
About this Structure
7RSA is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure of phosphate-free ribonuclease A refined at 1.26 A., Wlodawer A, Svensson LA, Sjolin L, Gilliland GL, Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:3401445
Page seeded by OCA on Mon Mar 31 05:44:44 2008
