8mht
From Proteopedia
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|PDB= 8mht |SIZE=350|CAPTION= <scene name='initialview01'>8mht</scene>, resolution 2.76Å | |PDB= 8mht |SIZE=350|CAPTION= <scene name='initialview01'>8mht</scene>, resolution 2.76Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=DU:2'-DEOXYURIDINE-5'-MONOPHOSPHATE'>DU</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8mht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8mht OCA], [http://www.ebi.ac.uk/pdbsum/8mht PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8mht RCSB]</span> | ||
}} | }} | ||
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[[Category: Horton, J R.]] | [[Category: Horton, J R.]] | ||
[[Category: Roberts, R J.]] | [[Category: Roberts, R J.]] | ||
| - | [[Category: SAH]] | ||
[[Category: complex (methyltransferase/ dna)]] | [[Category: complex (methyltransferase/ dna)]] | ||
[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:45:34 2008'' |
Revision as of 02:45, 31 March 2008
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| , resolution 2.76Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Activity: | Deleted entry, with EC number 2.1.1.73 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX
Overview
Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.
About this Structure
8MHT is a Single protein structure of sequence from Haemophilus haemolyticus. Full crystallographic information is available from OCA.
Reference
Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base., O'Gara M, Horton JR, Roberts RJ, Cheng X, Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:9783745
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