6f8h
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==antitoxin GraA== | |
| + | <StructureSection load='6f8h' size='340' side='right' caption='[[6f8h]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6f8h]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F8H FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f8h OCA], [http://pdbe.org/6f8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f8h RCSB], [http://www.ebi.ac.uk/pdbsum/6f8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f8h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The graTA operon from Pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in solution and crystallizes in space group P21, with unit-cell parameters a = 66.9, b = 48.9, c = 62.7 A, beta = 92.6 degrees . The crystals are likely to contain two GraA dimers in the asymmetric unit and diffract to 1.9 A resolution. The GraTA complex forms a heterotetramer in solution. Crystals of the GraTA complex diffracted to 2.2 A resolution and are most likely to contain a single heterotetrameric GraTA complex in the asymmetric unit. They belong to space group P41 or P43, with unit-cell parameters a = b = 56.0, c = 128.2 A. The GraA-operator complex consists of a 33 bp operator region that binds two GraA dimers. It crystallizes in space group P31 or P32, with unit-cell parameters a = b = 105.6, c = 149.9 A. These crystals diffract to 3.8 A resolution. | ||
| - | + | Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator.,Talavera A, Tamman H, Ainelo A, Hadaei S, Garcia-Pino A, Horak R, Konijnenberg A, Loris R Acta Crystallogr F Struct Biol Commun. 2017 Aug 1;73(Pt 8):455-462. doi:, 10.1107/S2053230X17009438. Epub 2017 Jul 26. PMID:28777088<ref>PMID:28777088</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 6f8h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Loris, R]] | [[Category: Loris, R]] | ||
[[Category: Talavera, A]] | [[Category: Talavera, A]] | ||
| + | [[Category: Antitoxin]] | ||
| + | [[Category: Graa]] | ||
| + | [[Category: Higa]] | ||
Revision as of 08:17, 30 January 2019
antitoxin GraA
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Categories: Loris, R | Talavera, A | Antitoxin | Graa | Higa
