2zbk
From Proteopedia
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] </span> | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] </span> | ||
|GENE= top6A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 Sulfolobus shibatae]), top6B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 Sulfolobus shibatae]) | |GENE= top6A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 Sulfolobus shibatae]), top6B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 Sulfolobus shibatae]) | ||
| - | |DOMAIN= | + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00223 TOPRIM_TopoIIB_SPO], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam04406 TP6A_N], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK04342 PRK04342], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK05179 rpsM], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG1389 COG1389], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00823 TopoIIB_Trans], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00075 HATPase_c]</span> |
|RELATEDENTRY= | |RELATEDENTRY= | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbk OCA], [http://www.ebi.ac.uk/pdbsum/2zbk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zbk RCSB]</span> | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbk OCA], [http://www.ebi.ac.uk/pdbsum/2zbk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zbk RCSB]</span> | ||
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'''Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms''' | '''Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms''' | ||
| + | |||
| + | ==Overview== | ||
| + | DNA topoisomerases resolve DNA topological problems created during transcription, replication, and recombination. These ubiquitous enzymes are essential for cell viability and are highly potent targets for the development of antibacterial and antitumoral drugs. Type II enzymes catalyze the transfer of a DNA duplex through another one in an ATP-dependent mechanism. Because of its small size and sensitivity to antitumoral drugs, the archaeal DNA topoisomerase VI, a type II enzyme, is an excellent model for gaining further understanding of the organization and mechanism of these enzymes. We present the crystal structure of intact DNA topoisomerase VI bound to radicicol, an inhibitor of human topo II, and compare it to the conformation of the apo-protein as determined by small-angle X-ray scattering in solution. This structure, combined with a wealth of experimental data gathered on these enzymes, allows us to propose a structural model for the two-gate DNA transfer mechanism. | ||
==About this Structure== | ==About this Structure== | ||
2ZBK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_shibatae Sulfolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBK OCA]. | 2ZBK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_shibatae Sulfolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBK OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Crystal Structure of an Intact Type II DNA Topoisomerase: Insights into DNA Transfer Mechanisms., Graille M, Cladiere L, Durand D, Lecointe F, Gadelle D, Quevillon-Cheruel S, Vachette P, Forterre P, van Tilbeurgh H, Structure. 2008 Mar;16(3):360-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18334211 18334211] | ||
[[Category: DNA topoisomerase (ATP-hydrolyzing)]] | [[Category: DNA topoisomerase (ATP-hydrolyzing)]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: nucleotide-binding]] | [[Category: nucleotide-binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:32:34 2008'' |
Revision as of 08:32, 2 April 2008
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| , resolution 3.560Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , and | ||||||
| Ligands: | |||||||
| Gene: | top6A (Sulfolobus shibatae), top6B (Sulfolobus shibatae) | ||||||
| Activity: | DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 | ||||||
| Domains: | TOPRIM_TopoIIB_SPO, TP6A_N, PRK04342, rpsM, COG1389, TopoIIB_Trans, HATPase_c | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms
Overview
DNA topoisomerases resolve DNA topological problems created during transcription, replication, and recombination. These ubiquitous enzymes are essential for cell viability and are highly potent targets for the development of antibacterial and antitumoral drugs. Type II enzymes catalyze the transfer of a DNA duplex through another one in an ATP-dependent mechanism. Because of its small size and sensitivity to antitumoral drugs, the archaeal DNA topoisomerase VI, a type II enzyme, is an excellent model for gaining further understanding of the organization and mechanism of these enzymes. We present the crystal structure of intact DNA topoisomerase VI bound to radicicol, an inhibitor of human topo II, and compare it to the conformation of the apo-protein as determined by small-angle X-ray scattering in solution. This structure, combined with a wealth of experimental data gathered on these enzymes, allows us to propose a structural model for the two-gate DNA transfer mechanism.
About this Structure
2ZBK is a Protein complex structure of sequences from Sulfolobus shibatae. Full crystallographic information is available from OCA.
Reference
Crystal Structure of an Intact Type II DNA Topoisomerase: Insights into DNA Transfer Mechanisms., Graille M, Cladiere L, Durand D, Lecointe F, Gadelle D, Quevillon-Cheruel S, Vachette P, Forterre P, van Tilbeurgh H, Structure. 2008 Mar;16(3):360-70. PMID:18334211
Page seeded by OCA on Wed Apr 2 11:32:34 2008
Categories: DNA topoisomerase (ATP-hydrolyzing) | Protein complex | Sulfolobus shibatae | Cladiere, L. | Durand, D. | Forterre, P. | Graille, M. | Lecointe, F. | Tilbeurgh, H van. | Atp-binding | Atpase | Decatenation | Dna binding protein | Dna topoisomerase | Dna-binding | Drug design | Magnesium | Metal-binding | Nucleotide-binding
