3bjx
From Proteopedia
OCA (Talk | contribs)
(New page: 200px {{Structure |PDB= 3bjx |SIZE=350|CAPTION= <scene name='initialview01'>3bjx</scene>, resolution 2.300Å |SITE= <scene name='pdbsite=AC1:So4+Binding+Site...)
Next diff →
Revision as of 08:59, 2 April 2008
| |||||||
| , resolution 2.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , , , , and | ||||||
| Ligands: | , | ||||||
| Gene: | dehI (Pseudomonas putida) | ||||||
| Activity: | (S)-2-haloacid dehalogenase, with EC number 3.8.1.2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3
Overview
Haloacid dehalogenases catalyse the removal of halides from organic haloacids and are of interest for bioremediation and for their potential use in the synthesis of industrial chemicals. We present the crystal structure of the homodimer DehI from Pseudomonas putida strain PP3, the first structure of a group I alpha-haloacid dehalogenase that can process both l- and d-substrates. The structure shows that the DehI monomer consists of two domains of approximately 130 amino acids that have approximately 16% sequence identity yet adopt virtually identical and unique folds that form a pseudo-dimer. Analysis of the active site reveals the likely binding mode of both l- and d-substrates with respect to key catalytic residues. Asp189 is predicted to activate a water molecule for nucleophilic attack of the substrate chiral centre resulting in an inversion of configuration of either l- or d-substrates in contrast to d-only enzymes. These details will assist with future bioengineering of dehalogenases.
About this Structure
3BJX is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
The Crystal Structure of DehI Reveals a New alpha-Haloacid Dehalogenase Fold and Active-Site Mechanism., Schmidberger JW, Wilce JA, Weightman AJ, Whisstock JC, Wilce MC, J Mol Biol. 2008 Feb 29;. PMID:18353360
Page seeded by OCA on Wed Apr 2 11:59:16 2008
