6ivz
From Proteopedia
(Difference between revisions)
m (Protected "6ivz" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of 5A ScFv complexed with YFV-China sE in postfusion state== | |
| + | <StructureSection load='6ivz' size='340' side='right' caption='[[6ivz]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ivz]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IVZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ivz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ivz OCA], [http://pdbe.org/6ivz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ivz RCSB], [http://www.ebi.ac.uk/pdbsum/6ivz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ivz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Yellow fever virus (YFV), a deadly human pathogen, is the prototype of the genus Flavivirus. Recently, YFV re-emerged in Africa and Brazil, leading to hundreds of deaths, with some cases imported to China. Prophylactic or therapeutic countermeasures are urgently needed. Previously, several human monoclonal antibodies against YFV were screened out by phage display. Here, we find that one of them, 5A, exhibits high neutralizing potency and good protection. Crystallographic analysis of the YFV envelope (E) protein in its pre- and post-fusion states shows conformations similar to those observed in other E proteins of flaviviruses. Furthermore, the structures of 5A in complex with the E protein in both states are resolved, revealing an invariant recognition site. Structural analysis and functional data suggest that 5A has high neutralization potency because it interferes with virus entry by preventing both virus attachment and fusion. These findings will be instrumental for immunogen or inhibitor design. | ||
| - | + | Double Lock of a Human Neutralizing and Protective Monoclonal Antibody Targeting the Yellow Fever Virus Envelope.,Lu X, Xiao H, Li S, Pang X, Song J, Liu S, Cheng H, Li Y, Wang X, Huang C, Guo T, Ter Meulen J, Daffis S, Yan J, Dai L, Rao Z, Klenk HD, Qi J, Shi Y, Gao GF Cell Rep. 2019 Jan 8;26(2):438-446.e5. doi: 10.1016/j.celrep.2018.12.065. PMID:30625326<ref>PMID:30625326</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6ivz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Li, S H]] | ||
| + | [[Category: Lu, X S]] | ||
| + | [[Category: Pang, X F]] | ||
| + | [[Category: Xiao, H X]] | ||
| + | [[Category: Envelope protein]] | ||
| + | [[Category: Flavivirus]] | ||
| + | [[Category: Neutralizing monoclonal antibody]] | ||
| + | [[Category: Viral protein]] | ||
| + | [[Category: Viral protein-immune system complex]] | ||
| + | [[Category: Yellow fever virus]] | ||
Revision as of 09:33, 13 February 2019
Crystal structure of 5A ScFv complexed with YFV-China sE in postfusion state
| |||||||||||
