6dyo
From Proteopedia
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<StructureSection load='6dyo' size='340' side='right' caption='[[6dyo]], [[Resolution|resolution]] 2.84Å' scene=''> | <StructureSection load='6dyo' size='340' side='right' caption='[[6dyo]], [[Resolution|resolution]] 2.84Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6dyo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DYO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dyo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DYO FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyo OCA], [http://pdbe.org/6dyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dyo RCSB], [http://www.ebi.ac.uk/pdbsum/6dyo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyo ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyo OCA], [http://pdbe.org/6dyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dyo RCSB], [http://www.ebi.ac.uk/pdbsum/6dyo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The protein Ebony from Drosophila melanogaster plays a central role in the regulation of histamine and dopamine in various tissues through condensation of these amines with beta-alanine. Ebony is a rare example of a nonribosomal peptide synthetase (NRPS) from a higher eukaryote and contains a C-terminal sequence that does not correspond to any previously characterized NRPS domain. We have structurally characterized this C-terminal domain and have discovered that it adopts the aryl-alkylamine-N-acetyl transferase (AANAT) fold, which is unprecedented in NRPS biology. Through analysis of ligand-bound structures, activity assays, and binding measurements, we have determined how this atypical condensation domain is able to provide selectivity for both the carrier protein-bound amino acid and the amine substrates, a situation that remains unclear for standard condensation domains identified to date from NRPS assembly lines. These results demonstrate that the C terminus of Ebony encodes a eukaryotic example of an alternative type of NRPS condensation domain; they also illustrate how the catalytic components of such assembly lines are significantly more diverse than a minimal set of conserved functional domains. | ||
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| + | Drosophila melanogaster nonribosomal peptide synthetase Ebony encodes an atypical condensation domain.,Izore T, Tailhades J, Hansen MH, Kaczmarski JA, Jackson CJ, Cryle MJ Proc Natl Acad Sci U S A. 2019 Jan 31. pii: 1811194116. doi:, 10.1073/pnas.1811194116. PMID:30705105<ref>PMID:30705105</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6dyo" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Drome]] | ||
[[Category: Cryle, M J]] | [[Category: Cryle, M J]] | ||
[[Category: Hansen, M H]] | [[Category: Hansen, M H]] | ||
Revision as of 09:46, 13 February 2019
C-terminal condensation domain of Ebony in complex with L-Dopamine
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