CzrA

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Zn<sup>+2</sup> binding is driven by a large [https://en.wikipedia.org/wiki/Entropy entropic] gain <ref>DOI:10.1021/ja906131b</ref>. Water molecules around the metal ion and Czr A protein are displaced, and gain greater freedom. This gain in entropy allows Zn<sup>+2</sup> to bind to Czr A with reasonable affinity and speed in vivo. The zinc<sup>+2</sup> ion forms a tetrahedral complex with the four residues (Figure 4). Other metal ions that may form a tetrahedral complex will have some affinity for Czr A; however, the metal binding pocket of Czr A has been optimized to bind Zn<sup>+2</sup> with the highest affinity.
Zn<sup>+2</sup> binding is driven by a large [https://en.wikipedia.org/wiki/Entropy entropic] gain <ref>DOI:10.1021/ja906131b</ref>. Water molecules around the metal ion and Czr A protein are displaced, and gain greater freedom. This gain in entropy allows Zn<sup>+2</sup> to bind to Czr A with reasonable affinity and speed in vivo. The zinc<sup>+2</sup> ion forms a tetrahedral complex with the four residues (Figure 4). Other metal ions that may form a tetrahedral complex will have some affinity for Czr A; however, the metal binding pocket of Czr A has been optimized to bind Zn<sup>+2</sup> with the highest affinity.
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== 3D Structures of CzrA ==
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[[CzrA 3D structures]]
</StructureSection>
</StructureSection>

Current revision

The dimer Czr A

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References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43 18177-18182.
  2. Chakravorty DK, Wang B, Lee CW, Giedroc DP, Merz KM Jr. Simulations of allosteric motions in the zinc sensor CzrA. J Am Chem Soc. 2012 Feb 22;134(7):3367-76. doi: 10.1021/ja208047b. Epub 2011 Nov , 14. PMID:22007899 doi:http://dx.doi.org/10.1021/ja208047b
  3. MacPherson S, Larochelle M, Turcotte B. A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiol Mol Biol Rev. 2006 Sep;70(3):583-604. PMID:16959962 doi:http://dx.doi.org/10.1128/MMBR.00015-06
  4. Miller J, McLachlan AD, Klug A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 1985 Jun 4;4(6):1609-1614.
  5. Grossoehme NE, Giedroc DP. Energetics of allosteric negative coupling in the zinc sensor S. aureus CzrA. J Am Chem Soc. 2009 Dec 16;131(49):17860-70. doi: 10.1021/ja906131b. PMID:19995076 doi:http://dx.doi.org/10.1021/ja906131b


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