6hc5
From Proteopedia
(Difference between revisions)
m (Protected "6hc5" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with audouine== |
| + | <StructureSection load='6hc5' size='340' side='right' caption='[[6hc5]], [[Resolution|resolution]] 1.41Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6hc5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HC5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HC5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FXT:1-(5-carbamimidamidopentyl)guanidine'>FXT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hc5 OCA], [http://pdbe.org/6hc5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hc5 RCSB], [http://www.ebi.ac.uk/pdbsum/6hc5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hc5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We have designed structure-based ligands for the guanidine-II riboswitch that bind with enhanced affinity, exploiting the twin binding sites created by loop-loop interaction. We synthesized diguanidine species, comprising two guanidino groups covalently connected by Cn linkers where n = 4 or 5. Calorimetric and fluorescent analysis shows that these ligands bind with a ten-fold higher affinity to the riboswitch compared to guanidine. We determined X-ray crystal structures of the riboswitch bound to the new ligands, showing that the guanidino groups are bound to both nucleobases and backbone within the binding pockets, analogously to guanidine binding. The connecting chain passes through side openings in the binding pocket and traverses the minor groove of the RNA. The combination of the riboswitch loop-loop interaction and our novel ligands have potential applications in chemical biology. | ||
| - | + | Structure-guided design of a high affinity ligand for a riboswitch.,Huang L, Wang J, Wilson TJ, Lilley DMJ RNA. 2019 Jan 4. pii: rna.069567.118. doi: 10.1261/rna.069567.118. PMID:30609994<ref>PMID:30609994</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6hc5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Huang, L]] | ||
| + | [[Category: Lilley, D M.J]] | ||
| + | [[Category: Dimer]] | ||
| + | [[Category: Guanidine ii riboswitch]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Stem-loop]] | ||
| + | [[Category: Tetra loop]] | ||
Revision as of 06:43, 21 February 2019
The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with audouine
| |||||||||||
