7,8-diaminopelargonic acid synthase
From Proteopedia
(Difference between revisions)
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| - | <StructureSection load='1qj3' size='350' side='right' caption='Structure of E. coli 7,8-diaminopelargonic acid | + | <StructureSection load='1qj3' size='350' side='right' caption='Structure of E. coli 7,8-diaminopelargonic acid synthase complex with PLP, KAPA and Na+ ion (purple) (PDB code [[1qj3]]). ' scene='59/595793/Cv/1'> |
== Function == | == Function == | ||
| - | '''7,8-diaminopelargonic acid | + | '''7,8-diaminopelargonic acid synthase''' (DAPAS) is part of the biotin biosynthesis pathway. DAPAS catalyzes the amino transfer from S-adenosylmethionine to 7-keto-8-pelargonic acid (KAPA) to produce S-adenosyl-4-methylthio-2-oxobutanoate and 7,8-diaminopelargonic acid. DAPAS is a pyridoxal phosphate (PLP)-dependent enzyme.<ref>PMID:12379100</ref> '''Bifunctional DAPAS/dethiobiotin synthase''' (DAPS/DTBS) catalyzes the above reaction and the conversion of 7,8-diaminonanoate, ATP and CO2 to ADP, phosphate and dethiobiotin. |
*<scene name='59/595793/Cv/7'>KAPA binding site</scene>. | *<scene name='59/595793/Cv/7'>KAPA binding site</scene>. | ||
*<scene name='59/595793/Cv/8'>PLP binding site</scene> (residues of chain B are in yellow and labeled (B). | *<scene name='59/595793/Cv/8'>PLP binding site</scene> (residues of chain B are in yellow and labeled (B). | ||
*<scene name='59/595793/Cv/9'>KAPA/PLP binding sites together</scene> (PDB code [[1qj3]]).<ref>PMID:10452893</ref> | *<scene name='59/595793/Cv/9'>KAPA/PLP binding sites together</scene> (PDB code [[1qj3]]).<ref>PMID:10452893</ref> | ||
*<scene name='59/595793/Cv/10'>Na coordination site</scene>. | *<scene name='59/595793/Cv/10'>Na coordination site</scene>. | ||
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| + | == 3D Structures of 7,8-diaminopelargonic acid synthase== | ||
| + | [[7,8-diaminopelargonic acid synthase 3D structures]] | ||
| + | |||
</StructureSection> | </StructureSection> | ||
| - | == 3D Structures of 7,8-diaminopelargonic acid | + | == 3D Structures of 7,8-diaminopelargonic acid synthase== |
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
{{#tree:id=OrganizedByTopic|openlevels=0| | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
| - | *7,8-diaminopelargonic acid | + | *7,8-diaminopelargonic acid synthase |
**[[1dty]], [[1qj5]] – EcDAPAS + PLP - ''Escherichia coli'' <br /> | **[[1dty]], [[1qj5]] – EcDAPAS + PLP - ''Escherichia coli'' <br /> | ||
**[[1mgv]], [[1s07]] – EcDAPAS (mutant) + PLP <br /> | **[[1mgv]], [[1s07]] – EcDAPAS (mutant) + PLP <br /> | ||
**[[3bv0]] – MtDAPAS (mutant) + PLP – ''Mycobacterium tuberculosis''<br /> | **[[3bv0]] – MtDAPAS (mutant) + PLP – ''Mycobacterium tuberculosis''<br /> | ||
| + | **[[5te2]] – MtDAPAS + PLP derivative<br /> | ||
**[[3dod]] – BsDAPAS + PLP – ''Bacillus subtilis''<br /> | **[[3dod]] – BsDAPAS + PLP – ''Bacillus subtilis''<br /> | ||
**[[3drd]] – BsDAPAS + PLP <br /> | **[[3drd]] – BsDAPAS + PLP <br /> | ||
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**[[4a0f]] – AtDAPAS (mutant) + PLP <br /> | **[[4a0f]] – AtDAPAS (mutant) + PLP <br /> | ||
| - | *7,8-diaminopelargonic acid | + | *7,8-diaminopelargonic acid synthase complex |
**[[1qj3]] – EcDAPAS + PLP + KAPA<br /> | **[[1qj3]] – EcDAPAS + PLP + KAPA<br /> | ||
| Line 33: | Line 38: | ||
**[[4cxq]] – MtDAPAS + PLP + KAPA<br /> | **[[4cxq]] – MtDAPAS + PLP + KAPA<br /> | ||
**[[3lv2]] – MtDAPAS + PLP + sinefungin<br /> | **[[3lv2]] – MtDAPAS + PLP + sinefungin<br /> | ||
| - | **[[3tft]], [[3tfu]] – MtDAPAS + PLP + inhibitor<br /> | + | **[[3tft]], [[3tfu]], [[4w1v]], [[4w1w]], [[4w1x]], [[4wya]], [[4wyc]], [[4wye]], [[4wyf]], [[4wyg]], [[4xew]], [[4xjl]], [[4xjm]], [[4xjo]], [[4xjp]], [[5kgt]], [[5kgs]] – MtDAPAS + PLP + inhibitor<br /> |
**[[4mqp]], [[4mqq]], [[4mqr]] – MtDAPAS + inhibitor<br /> | **[[4mqp]], [[4mqq]], [[4mqr]] – MtDAPAS + inhibitor<br /> | ||
| - | *Bifunctional 7,8-diaminopelargonic acid | + | *Bifunctional 7,8-diaminopelargonic acid synthase/dethiobiotin synthase |
**[[4a0f]], [[4a0g]] – AtDAPAS + PLP <br /> | **[[4a0f]], [[4a0g]] – AtDAPAS + PLP <br /> | ||
**[[4a0r]] – AtDAPAS + PLP + dethiobiotin<br /> | **[[4a0r]] – AtDAPAS + PLP + dethiobiotin<br /> | ||
**[[4a0h]] – AtDAPAS + PLP + keto-aminopelargonic acid<br /> | **[[4a0h]] – AtDAPAS + PLP + keto-aminopelargonic acid<br /> | ||
| + | **[[5uc7]] - DAPAS + PLP derivative – ''Citrobacter rodentium''<br /> | ||
}} | }} | ||
== References == | == References == | ||
Revision as of 10:01, 21 February 2019
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3D Structures of 7,8-diaminopelargonic acid synthase
Updated on 21-February-2019
References
- ↑ Eliot AC, Sandmark J, Schneider G, Kirsch JF. The dual-specific active site of 7,8-diaminopelargonic acid synthase and the effect of the R391A mutation. Biochemistry. 2002 Oct 22;41(42):12582-9. PMID:12379100
- ↑ Kack H, Sandmark J, Gibson K, Schneider G, Lindqvist Y. Crystal structure of diaminopelargonic acid synthase: evolutionary relationships between pyridoxal-5'-phosphate-dependent enzymes. J Mol Biol. 1999 Aug 27;291(4):857-76. PMID:10452893 doi:http://dx.doi.org/10.1006/jmbi.1999.2997
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