Abrin

From Proteopedia

(Difference between revisions)

Revision as of 08:10, 25 February 2019

Abrin

1 Introduction
2 Structure
3 Medical Potential
4 Negative Effects/Treatment
5 Bioterrorism/Prevention
6 3D structures of abrin

Introduction

Abrus precatorius (or rosary peas) contain the protein abrin. Rosary peas are red and oval-shaped with a black edge towards one end. In some cultures, they are used to make beaded jewelry. Abrin can exhibit beneficial uses for medical research, but it can also be destructive if it directly enters an organism’s body because of its toxic properties.

Structure

Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains: and which are connected by a disulfide bond. Both of the chains are . The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase ^[1]. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA^[2]. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.

Medical Potential

Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression" ^[1] which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells ^[2].

Negative Effects/Treatment

If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms ^[3] Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently^[4].

Bioterrorism/Prevention

Abrin is very similar to ricin because of its structure and functions. While abrin hasn’t been used for bioterrorism, there have been multiple occasions of ricin misuse. For example, in London of 1978, Georgi Markov was attacked by a man with an umbrella, who was employed by the Bulgarian secret service. The umbrella was designed to release a piece ricin which ended up lodging into Markov's skin. Markov died three days after being exposed to the toxin of ricin ^[5]. Other acts of bioterrorism have included envelopes with the exposure to ricin being mailed to government officials including the President. Since then a vaccine has been created to suppress the effects of ricin poisoning. However, there is still a potential for the use of abrin in a bioterrorism attack. As of right now, they are in development of a vaccine and have learned in one study that a rATB vaccine will possibly rid the symptoms of abrin poisoning^[6].

3D structures of abrin

Abrin 3D structures

3D structures of abrin

Updated on 25-February-2019

1abr, 5z37 – IlAbr-A – Indian licorice
5z3i – IlAbr-A + adenine
5z3j – IlAbr-A + nicotinamide

References

↑ ^1.0 ^1.1 Yu Y, Yang R, Zhao X, Qin D, Liu Z, Liu F, Song X, Li L, Feng R, Gao N. Abrin P2 suppresses proliferation and induces apoptosis of colon cancer cells via mitochondrial membrane depolarization and caspase activation. Acta Biochim Biophys Sin (Shanghai). 2016 May;48(5):420-9. doi:, 10.1093/abbs/gmw023. Epub 2016 Apr 6. PMID:27055473 doi:http://dx.doi.org/10.1093/abbs/gmw023
↑ ^2.0 ^2.1 Wooten JV, Pittman CT, Blake TA, Thomas JD, Devlin JJ, Higgerson RA, Johnson RC. A case of abrin toxin poisoning, confirmed via quantitation of L-abrine (N-methyl-L-tryptophan) biomarker. J Med Toxicol. 2014 Dec;10(4):392-4. doi: 10.1007/s13181-013-0377-9. PMID:24522983 doi:http://dx.doi.org/10.1007/s13181-013-0377-9
↑ Alhamdani M, Brown B, Narula P. Abrin poisoning in an 18-month-old child. Am J Case Rep. 2015 Mar 10;16:146-8. doi: 10.12659/AJCR.892917. PMID:25754813 doi:http://dx.doi.org/10.12659/AJCR.892917
↑ Huang J, Zhang W, Li X, Feng S, Ye G, Wei H, Gong X. Acute abrin poisoning treated with continuous renal replacement therapy and hemoperfusion successfully: A case report. Medicine (Baltimore). 2017 Jul;96(27):e7423. doi: 10.1097/MD.0000000000007423. PMID:28682903 doi:http://dx.doi.org/10.1097/MD.0000000000007423
↑ "Facts About Abrin.” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 18 Nov. 2015, emergency.cdc.gov/agent/abrin/basics/facts.asp.
↑ Wang J, Gao S, Xin W, Kang L, Xu N, Zhang T, Liu W, Wang J. A novel recombinant vaccine protecting mice against abrin intoxication. Hum Vaccin Immunother. 2015;11(6):1361-7. doi: 10.1080/21645515.2015.1008879. PMID:26086588 doi:http://dx.doi.org/10.1080/21645515.2015.1008879

Proteopedia Page Contributors and Editors (what is this?)

Jason Telford, Emily Street, Michal Harel, Karsten Theis

Retrieved from "http://52.214.119.220/wiki/index.php/Abrin"

Category: Topic Page

@@ Line 12: / Line 12: @@
 == Bioterrorism/Prevention ==
 Abrin is very similar to ricin because of its structure and functions. While abrin hasn’t been used for bioterrorism, there have been multiple occasions of ricin misuse. For example, in London of 1978, Georgi Markov was attacked by a man with an umbrella, who was employed by the Bulgarian secret service. The umbrella was designed to release a piece ricin which ended up lodging into Markov's skin. Markov died three days after being exposed to the toxin of ricin <ref>"Facts About Abrin.” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 18 Nov. 2015, emergency.cdc.gov/agent/abrin/basics/facts.asp.</ref>. Other acts of bioterrorism have included envelopes with the exposure to ricin being mailed to government officials including the President. Since then a vaccine has been created to suppress the effects of ricin poisoning. However, there is still a potential for the use of abrin in a bioterrorism attack. As of right now, they are in development of a vaccine and have learned in one study that a rATB vaccine will possibly rid the symptoms of abrin poisoning<ref>doi:10.1080/21645515.2015.1008879</ref>.
+==3D structures of abrin==
+[[Abrin 3D structures]]
 </StructureSection>

Abrin

From Proteopedia

Revision as of 08:10, 25 February 2019

Abrin

Contents

Introduction

Structure

Medical Potential

Negative Effects/Treatment

Bioterrorism/Prevention

3D structures of abrin

3D structures of abrin

References

Proteopedia Page Contributors and Editors (what is this?)

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