6f9o
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5== | |
| + | <StructureSection load='6f9o' size='340' side='right' caption='[[6f9o]], [[Resolution|resolution]] 1.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6f9o]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F9O FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f9o OCA], [http://pdbe.org/6f9o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f9o RCSB], [http://www.ebi.ac.uk/pdbsum/6f9o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f9o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/DHMA_PSYCK DHMA_PSYCK]] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons.[HAMAP-Rule:MF_01230] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A. | ||
| - | + | Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.,Tratsiak K, Degtjarik O, Drienovska I, Chrast L, Rezacova P, Kuty M, Chaloupkova R, Damborsky J, Kuta Smatanova I Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):683-8. doi:, 10.1107/S1744309113012979. Epub 2013 May 25. PMID:23722854<ref>PMID:23722854</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6f9o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Haloalkane dehalogenase]] | ||
| + | [[Category: Brynda, J]] | ||
| + | [[Category: Chaloupkova, R]] | ||
| + | [[Category: Damborsky, J]] | ||
| + | [[Category: Drienovska, I]] | ||
| + | [[Category: Kuty, M]] | ||
| + | [[Category: Pachl, P]] | ||
| + | [[Category: Prudnikova, T]] | ||
| + | [[Category: Rezacova, P]] | ||
| + | [[Category: Smatanova, I Kuta]] | ||
| + | [[Category: Tratsiak, K]] | ||
| + | [[Category: 1-bromohexane and 1]] | ||
| + | [[Category: Domain]] | ||
| + | [[Category: 3-dibromopropane]] | ||
| + | [[Category: Alpha/beta-hydrolase fold enzyme]] | ||
| + | [[Category: Hld]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Microbial enzyme]] | ||
| + | [[Category: The highest activity towards 1-bromobutane]] | ||
Revision as of 15:17, 27 February 2019
Crystal structure of cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5
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Categories: Haloalkane dehalogenase | Brynda, J | Chaloupkova, R | Damborsky, J | Drienovska, I | Kuty, M | Pachl, P | Prudnikova, T | Rezacova, P | Smatanova, I Kuta | Tratsiak, K | 1-bromohexane and 1 | Domain | 3-dibromopropane | Alpha/beta-hydrolase fold enzyme | Hld | Hydrolase | Microbial enzyme | The highest activity towards 1-bromobutane
