6hol

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m (Protected "6hol" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 6hol is ON HOLD
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==Structure of ATG14 LIR motif bound to GABARAPL1==
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<StructureSection load='6hol' size='340' side='right' caption='[[6hol]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6hol]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HOL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hol OCA], [http://pdbe.org/6hol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hol RCSB], [http://www.ebi.ac.uk/pdbsum/6hol PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hol ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/GBRL1_HUMAN GBRL1_HUMAN]] Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles.<ref>PMID:16431922</ref> <ref>PMID:20404487</ref> [[http://www.uniprot.org/uniprot/BAKOR_HUMAN BAKOR_HUMAN]] Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604). Modulates the hepatic lipid metabolism (By similarity).[UniProtKB:Q8CDJ3]<ref>PMID:18843052</ref> <ref>PMID:19050071</ref> <ref>PMID:19270696</ref> <ref>PMID:20713597</ref> <ref>PMID:23878393</ref> <ref>PMID:25686604</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The latter contains PIK3C3/VPS34, PIK3R4/VPS15, BECN1/Beclin 1 and ATG14 and phosphorylates phosphatidylinositol to generate phosphatidylinositol 3-phosphate (PtdIns3P). Here, we show that PIK3C3, BECN1 and ATG14 contain functional LIR motifs and interact with the Atg8-family proteins with a preference for GABARAP and GABARAPL1. High resolution crystal structures of the functional LIR motifs of these core components of PtdIns3K-C1were obtained. Variation in hydrophobic pocket 2 (HP2) may explain the specificity for the GABARAP family. Mutation of the LIR motif in ATG14 did not prevent formation of the PtdIns3K-C1 complex, but blocked colocalization with MAP1LC3B/LC3B and impaired mitophagy. The ULK-mediated phosphorylation of S29 in ATG14 was strongly dependent on a functional LIR motif in ATG14. GABARAP-preferring LIR motifs in PIK3C3, BECN1 and ATG14 may, via coincidence detection, contribute to scaffolding of PtdIns3K-C1 on membranes for efficient autophagosome formation.
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Authors: Mouilleron, S., Birgisdottir, A.B., Bhujbal, Z., Wirth, M., Sjottem, E., Evjen, G., Zhang, W., Lee, R., O'Reilly, N., Tooze, S., Lamark, T., Johansen, T.
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Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs.,Birgisdottir AB, Mouilleron S, Bhujabal Z, Wirth M, Sjottem E, Evjen G, Zhang W, Lee R, O'Reilly N, Tooze SA, Lamark T, Johansen T Autophagy. 2019 Feb 15. doi: 10.1080/15548627.2019.1581009. PMID:30767700<ref>PMID:30767700</ref>
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Description: Structure of ATG14 LIR motif bound to GABARAPL1
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Lamark, T]]
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<div class="pdbe-citations 6hol" style="background-color:#fffaf0;"></div>
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[[Category: Lee, R]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bhujbal, Z]]
[[Category: Bhujbal, Z]]
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[[Category: O'Reilly, N]]
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[[Category: Birgisdottir, A B]]
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[[Category: Tooze, S]]
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[[Category: Evjen, G]]
[[Category: Evjen, G]]
[[Category: Johansen, T]]
[[Category: Johansen, T]]
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[[Category: Sjottem, E]]
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[[Category: Lamark, T]]
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[[Category: Lee, R]]
[[Category: Mouilleron, S]]
[[Category: Mouilleron, S]]
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[[Category: Zhang, W]]
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[[Category: Reilly, N O]]
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[[Category: Sjottem, E]]
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[[Category: Tooze, S]]
[[Category: Wirth, M]]
[[Category: Wirth, M]]
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[[Category: Birgisdottir, A.B]]
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[[Category: Zhang, W]]
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[[Category: Atg8]]
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[[Category: Autophagy]]
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[[Category: Lir]]
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[[Category: Signaling protein]]

Revision as of 15:22, 27 February 2019

Structure of ATG14 LIR motif bound to GABARAPL1

6hol, resolution 1.40Å

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