6j4n
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of papua new guinea MBL-1(PNGM-1) native== | |
| + | <StructureSection load='6j4n' size='340' side='right' caption='[[6j4n]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6j4n]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J4N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J4N FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j4n OCA], [http://pdbe.org/6j4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j4n RCSB], [http://www.ebi.ac.uk/pdbsum/6j4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j4n ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Metallo-beta-lactamases (MBLs) are present in major Gram-negative pathogens and environmental species, and pose great health risks because of their ability to hydrolyze the beta-lactam rings of antibiotics such as carbapenems. PNGM-1 was the first reported case of a subclass B3 MBL protein that was identified from a metagenomic library from deep-sea sediments that predate the antibiotic era. In this study, PNGM-1 was overexpressed, purified and crystallized. Crystals of native and selenomethionine-substituted PNGM-1 diffracted to 2.10 and 2.30 A resolution, respectively. Both the native and the selenomethionine-labelled PNGM-1 crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 122, b = 83, c = 163 A, beta = 110 degrees . Matthews coefficient (VM) calculations suggested the presence of 6-10 molecules in the asymmetric unit, corresponding to a solvent content of approximately 31-58%. Structure determination is currently in progress. | ||
| - | + | The novel metallo-beta-lactamase PNGM-1 from a deep-sea sediment metagenome: crystallization and X-ray crystallographic analysis.,Park KS, Hong MK, Jeon JW, Kim JH, Jeon JH, Lee JH, Kim TY, Karim AM, Malik SK, Kang LW, Lee SH Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):644-649. doi:, 10.1107/S2053230X18012268. Epub 2018 Sep 19. PMID:30279316<ref>PMID:30279316</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Hong, M | + | <div class="pdbe-citations 6j4n" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Kang, L | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Lee, | + | </StructureSection> |
| - | [[Category: | + | [[Category: Hong, M K]] |
| - | [[Category: Park, Y | + | [[Category: Jeon, J H]] |
| + | [[Category: Kang, L W]] | ||
| + | [[Category: Lee, J H]] | ||
| + | [[Category: Lee, S H]] | ||
| + | [[Category: Park, K S]] | ||
| + | [[Category: Park, Y S]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Metagenomic research]] | ||
| + | [[Category: Metallo beta lactamse]] | ||
| + | [[Category: Zinc binding motif]] | ||
Revision as of 15:27, 27 February 2019
Structure of papua new guinea MBL-1(PNGM-1) native
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