Adenosine dimethyltransferase
From Proteopedia
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Among methyltransferases, KsgA and the reaction it catalyzes are conserved throughout evolution. However, the specifics of substrate recognition by the enzyme remain unknown. Here we report structures of <scene name='59/597431/Cv/3'>Aquifex aeolicus KsgA, in its ligand-free form, in complex with RNA, and in complex with both RNA and S-adenosylhomocysteine</scene> (SAH, reaction product of cofactor S-adenosylmethionine), revealing critical structural information on KsgA-RNA and KsgA-SAH interactions. Moreover, the structures show how conformational changes that occur upon RNA binding create the cofactor-binding site. There are nine conserved functional motifs (motifs I-VIII and X) in KsgA. Prior to RNA binding, motifs I and VIII are flexible, each exhibiting two distinct conformations. Upon RNA binding, the two motifs become stabilized in one of these conformations, which is compatible with the binding of SAH. Motif X, which is also stabilized upon RNA binding, is directly involved in the binding of SAH.<ref>PMID:19278652</ref> | Among methyltransferases, KsgA and the reaction it catalyzes are conserved throughout evolution. However, the specifics of substrate recognition by the enzyme remain unknown. Here we report structures of <scene name='59/597431/Cv/3'>Aquifex aeolicus KsgA, in its ligand-free form, in complex with RNA, and in complex with both RNA and S-adenosylhomocysteine</scene> (SAH, reaction product of cofactor S-adenosylmethionine), revealing critical structural information on KsgA-RNA and KsgA-SAH interactions. Moreover, the structures show how conformational changes that occur upon RNA binding create the cofactor-binding site. There are nine conserved functional motifs (motifs I-VIII and X) in KsgA. Prior to RNA binding, motifs I and VIII are flexible, each exhibiting two distinct conformations. Upon RNA binding, the two motifs become stabilized in one of these conformations, which is compatible with the binding of SAH. Motif X, which is also stabilized upon RNA binding, is directly involved in the binding of SAH.<ref>PMID:19278652</ref> | ||
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| + | ==3D structures of adenosine dimethyltransferase== | ||
| + | [[Adenosine dimethyltransferase 3D structures]] | ||
</StructureSection> | </StructureSection> | ||
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**[[3uzu]] – KsgA – ''Burkholderia pseudomallei''<br /> | **[[3uzu]] – KsgA – ''Burkholderia pseudomallei''<br /> | ||
**[[4jxj]] – KsgA – ''Rickettsia bellii''<br /> | **[[4jxj]] – KsgA – ''Rickettsia bellii''<br /> | ||
| + | **[[6ifs]], [[6ifv]], [[6ifw]], [[6ifx]] – BsKsgA – ''Bacillus subtilis''<br /> | ||
*Adenosine dimethyltransferase complex | *Adenosine dimethyltransferase complex | ||
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**[[3r9x]] – AaKsgA + GDPNP + RNA + GTPase<br /> | **[[3r9x]] – AaKsgA + GDPNP + RNA + GTPase<br /> | ||
**[[4adv]] – EcKsgA + 30S ribosomal subunit – Cryo-EM<br /> | **[[4adv]] – EcKsgA + 30S ribosomal subunit – Cryo-EM<br /> | ||
| + | **[[4lf4]], [[4lf5]], [[4lf6]], [[4lf7]], [[4lf8]], [[4lf9]], [[4lfa]], [[4lfb]], [[4lfc]] – TtKsgA + 30S ribosomal subunit <br /> | ||
| + | **[[6ift]] – SbKsgA + SAM<br /> | ||
}} | }} | ||
Revision as of 10:59, 28 February 2019
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3D structures of adenosine dimethyltransferase
Updated on 28-February-2019
References
- ↑ Tu C, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X. Structural basis for binding of RNA and cofactor by a KsgA methyltransferase. Structure. 2009 Mar 11;17(3):374-85. PMID:19278652 doi:10.1016/j.str.2009.01.010
