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| - | | + | #REDIRECT [[6njg]] This PDB entry is obsolete and replaced by 6njg |
| - | ==Ubiquitin Variant in Complex with Binding Partner==
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| - | <StructureSection load='5ucl' size='340' side='right' caption='[[5ucl]], [[Resolution|resolution]] 2.35Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[5ucl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCL FirstGlance]. <br>
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| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ucl OCA], [http://pdbe.org/5ucl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ucl RCSB], [http://www.ebi.ac.uk/pdbsum/5ucl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ucl ProSAT]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [[http://www.uniprot.org/uniprot/VPS27_YEAST VPS27_YEAST]] Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC. Might also function as an alternate adapter in the COPIb clathrin-like coat.<ref>PMID:3062374</ref> <ref>PMID:1493335</ref> <ref>PMID:8649377</ref> <ref>PMID:9015300</ref> <ref>PMID:9265642</ref> <ref>PMID:11208109</ref> <ref>PMID:11416128</ref> <ref>PMID:12055639</ref> <ref>PMID:11872141</ref> <ref>PMID:12900393</ref> <ref>PMID:14581452</ref> <ref>PMID:15166140</ref> <ref>PMID:15107463</ref> <ref>PMID:15086794</ref> <ref>PMID:17101773</ref> <ref>PMID:17135292</ref>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Ubiquitin interacting motifs (UIMs) are short alpha-helices found in a number of eukaryotic proteins. UIMs interact weakly but specifically with ubiquitin conjugated to other proteins, and in so doing, mediate specific cellular signals. Here we used phage display to generate ubiquitin variants (UbVs) targeting the N-terminal UIM of the yeast Vps27 protein. Selections yielded UbV.v27.1, which recognized the cognate UIM with high specificity relative to other yeast UIMs and bound with an affinity more than two orders of magnitude higher than that of ubiquitin. Structural and mutational studies of the UbV.v27.1-UIM complex revealed the molecular details for the enhanced affinity and specificity of UbV.v27.1, and underscored the importance of changes at the binding interface as well as at positions that do not contact the UIM. Our study highlights the power of the phage display approach for selecting UbVs with unprecedented affinity and high selectivity for particular alpha-helical UIM domains within proteomes, and it establishes a general approach for the development of inhibitors targeting interactions of this type. This article is protected by copyright. All rights reserved.
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| - | Structural and functional characterization of a ubiquitin variant engineered for tight and specific binding to an alpha-helical ubiquitin interacting motif.,Manczyk N, Yates BP, Veggiani G, Ernst A, Sicheri F, Sidhu SS Protein Sci. 2017 Mar 9. doi: 10.1002/pro.3155. PMID:28276594<ref>PMID:28276594</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5ucl" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Human]]
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| - | [[Category: Manczyk, N]]
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| - | [[Category: Sicheri, F]]
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| - | [[Category: Protein binding]]
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| - | [[Category: Ubiquitin variant]]
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| - | [[Category: Uim]]
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