6mbc
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Human Bfl-1 in complex with the designed peptide dF4== | |
| + | <StructureSection load='6mbc' size='340' side='right' caption='[[6mbc]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6mbc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MBC FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mbc OCA], [http://pdbe.org/6mbc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mbc RCSB], [http://www.ebi.ac.uk/pdbsum/6mbc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mbc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/B2LA1_HUMAN B2LA1_HUMAN]] Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Understanding the relationship between protein sequence and structure well enough to design new proteins with desired functions is a longstanding goal in protein science. Here, we show that recurring tertiary structural motifs (TERMs) in the PDB provide rich information for protein-peptide interaction prediction and design. TERM statistics can be used to predict peptide binding energies for Bcl-2 family proteins as accurately as widely used structure-based tools. Furthermore, design using TERM energies (dTERMen) rapidly and reliably generates high-affinity peptide binders of anti-apoptotic proteins Bfl-1 and Mcl-1 with just 15%-38% sequence identity to any known native Bcl-2 family protein ligand. High-resolution structures of four designed peptides bound to their targets provide opportunities to analyze the strengths and limitations of the computational design method. Our results support dTERMen as a powerful approach that can complement existing tools for protein engineering. | ||
| - | + | Tertiary Structural Motif Sequence Statistics Enable Facile Prediction and Design of Peptides that Bind Anti-apoptotic Bfl-1 and Mcl-1.,Frappier V, Jenson JM, Zhou J, Grigoryan G, Keating AE Structure. 2019 Feb 12. pii: S0969-2126(19)30008-5. doi:, 10.1016/j.str.2019.01.008. PMID:30773399<ref>PMID:30773399</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Jenson, J | + | <div class="pdbe-citations 6mbc" style="background-color:#fffaf0;"></div> |
| - | [[Category: Keating, A | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Jenson, J M]] | ||
| + | [[Category: Keating, A E]] | ||
| + | [[Category: Anti-apoptotic bcl-2]] | ||
| + | [[Category: Apoptosis]] | ||
| + | [[Category: Design]] | ||
| + | [[Category: Inhibitor]] | ||
Revision as of 07:33, 6 March 2019
Human Bfl-1 in complex with the designed peptide dF4
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