6h3t
From Proteopedia
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==Schmallenberg Virus Glycoprotein Gc Head Domain in Complex with scFv 1C11== | ==Schmallenberg Virus Glycoprotein Gc Head Domain in Complex with scFv 1C11== | ||
| - | <StructureSection load='6h3t' size='340' side='right' caption='[[6h3t]], [[Resolution|resolution]] 2.84Å' scene=''> | + | <StructureSection load='6h3t' size='340' side='right' caption='[[6h3t]], [[Resolution|resolution]] 2.84Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6h3t]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6h3t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_schmallenberg_virus_(isolate_bovine/bh80/germany/2011) Bovine schmallenberg virus (isolate bovine/bh80/germany/2011)] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3T FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318464 Bovine Schmallenberg virus (isolate Bovine/BH80/Germany/2011)])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3t OCA], [http://pdbe.org/6h3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3t RCSB], [http://www.ebi.ac.uk/pdbsum/6h3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3t ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3t OCA], [http://pdbe.org/6h3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3t RCSB], [http://www.ebi.ac.uk/pdbsum/6h3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/GP_SBVBH GP_SBVBH]] Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity). | [[http://www.uniprot.org/uniprot/GP_SBVBH GP_SBVBH]] Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Orthobunyaviruses (OBVs) form a distinct genus of arthropod-borne bunyaviruses that can cause severe disease upon zoonotic transmission to humans. Antigenic drift or genome segment re-assortment have in the past resulted in new pathogenic OBVs, making them potential candidates for causing emerging zoonoses in the future. Low-resolution electron cryo-tomography studies have shown that OBV particles feature prominent trimeric spikes, but their molecular organization remained unknown. Here we report X-ray crystallography studies of four different OBVs showing that the spikes are formed by an N-terminal extension of the fusion glycoprotein Gc. Using Schmallenberg virus, a recently emerged OBV, we also show that the projecting spike is the major target of the neutralizing antibody response, and provide X-ray structures in complex with two protecting antibodies. We further show that immunization of mice with the spike domains elicits virtually sterilizing immunity, providing fundamental knowledge essential in the preparation for potential newly emerging OBV zoonoses. | ||
| + | |||
| + | Orthobunyavirus spike architecture and recognition by neutralizing antibodies.,Hellert J, Aebischer A, Wernike K, Haouz A, Brocchi E, Reiche S, Guardado-Calvo P, Beer M, Rey FA Nat Commun. 2019 Feb 20;10(1):879. doi: 10.1038/s41467-019-08832-8. PMID:30787296<ref>PMID:30787296</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6h3t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Lk3 transgenic mice]] | ||
[[Category: Aebischer, A]] | [[Category: Aebischer, A]] | ||
[[Category: Beer, M]] | [[Category: Beer, M]] | ||
Revision as of 08:25, 6 March 2019
Schmallenberg Virus Glycoprotein Gc Head Domain in Complex with scFv 1C11
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