6ikj
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of YfiB(F48S)== | |
| + | <StructureSection load='6ikj' size='340' side='right'caption='[[6ikj]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ikj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IKJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ikj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ikj OCA], [http://pdbe.org/6ikj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ikj RCSB], [http://www.ebi.ac.uk/pdbsum/6ikj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ikj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial cyclic-di-GMP (c-di-GMP) is an important messenger molecule that influences diverse cellular processes including motility, virulence and cytotoxicity systems, polysaccharide synthesis and biofilm formation. The YfiBNR tripartite signalling system in P. aeruginosa modulates the cellular c-di-GMP levels in response to signals received from the periplasm. In this study, we analyse the structures of activating mutants of the outer membrane protein YfiB that give rise to increased surface attachment and biofilm formation. The F48S and W55L mutants of YfiB(27-168) crystallize in the same dimeric arrangement as our previously reported YfiB structures that preclude complex formation with YfiR. The L43P mutant of YfiB(27-168) is monomeric and forms a stable complex with YfiR. The YfiB(L43P)-YfiR crystal structure reveals a dramatic rearrangement of the N-terminal fragment, which is implicated in increased YfiB activation and membrane attachment, upon YfiR binding. Comparison with our previous complex structure between YfiB(59-168) and YfiR reveals extensive interactions between the N-terminal fragment of YfiB (residues 35-55) and YfiR. | ||
| - | + | Structural analysis of activating mutants of YfiB from Pseudomonas aeruginosa PAO1.,Li S, Li T, Teng X, Lou X, Xu Y, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2018 Dec 2;506(4):997-1003. doi:, 10.1016/j.bbrc.2018.10.190. Epub 2018 Nov 4. PMID:30404734<ref>PMID:30404734</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 6ikj" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Bartlam, M]] | [[Category: Bartlam, M]] | ||
| + | [[Category: Li, S]] | ||
[[Category: Zhang, Q]] | [[Category: Zhang, Q]] | ||
| + | [[Category: Peptidoglycan binding]] | ||
| + | [[Category: Signaling protein]] | ||
| + | [[Category: Signalling system]] | ||
Revision as of 12:05, 13 March 2019
Crystal structure of YfiB(F48S)
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