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6imm
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of an alphavirus, Sindbis virus== | |
| + | <StructureSection load='6imm' size='340' side='right'caption='[[6imm]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6imm]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sindbis_virus Sindbis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IMM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IMM FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6imm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6imm OCA], [http://pdbe.org/6imm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6imm RCSB], [http://www.ebi.ac.uk/pdbsum/6imm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6imm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Alphaviruses are enveloped RNA viruses that contain several human pathogens. Due to intrinsic heterogeneity of alphavirus particles, a high resolution structure of the virion is currently lacking. Here we provide a 3.5 A cryo-EM structure of Sindbis virus, using block based reconstruction method that overcomes the heterogeneity problem. Our structural analysis identifies a number of conserved residues that play pivotal roles in the virus life cycle. We identify a hydrophobic pocket in the subdomain D of E2 protein that is stabilized by an unknown pocket factor near the viral membrane. Residues in the pocket are conserved in different alphaviruses. The pocket strengthens the interactions of the E1/E2 heterodimer and may facilitate virus assembly. Our study provides structural insights into alphaviruses that may inform the design of drugs and vaccines. | ||
| - | + | Implication for alphavirus host-cell entry and assembly indicated by a 3.5A resolution cryo-EM structure.,Chen L, Wang M, Zhu D, Sun Z, Ma J, Wang J, Kong L, Wang S, Liu Z, Wei L, He Y, Wang J, Zhang X Nat Commun. 2018 Dec 14;9(1):5326. doi: 10.1038/s41467-018-07704-x. PMID:30552337<ref>PMID:30552337</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6imm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sindbis virus]] | ||
| + | [[Category: Chen, L]] | ||
| + | [[Category: Ma, J]] | ||
| + | [[Category: Zhang, X]] | ||
| + | [[Category: Alphavirus]] | ||
| + | [[Category: Glycoprotein]] | ||
| + | [[Category: Virus]] | ||
Revision as of 12:05, 13 March 2019
Cryo-EM structure of an alphavirus, Sindbis virus
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Categories: Large Structures | Sindbis virus | Chen, L | Ma, J | Zhang, X | Alphavirus | Glycoprotein | Virus
