5gt7
From Proteopedia
(Difference between revisions)
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==Crystal Structure of Arg-bound CASTOR1== | ==Crystal Structure of Arg-bound CASTOR1== | ||
| - | <StructureSection load='5gt7' size='340' side='right' caption='[[5gt7]], [[Resolution|resolution]] 2.05Å' scene=''> | + | <StructureSection load='5gt7' size='340' side='right'caption='[[5gt7]], [[Resolution|resolution]] 2.05Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5gt7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GT7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5gt7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GT7 FirstGlance]. <br> | ||
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/GATL3_HUMAN GATL3_HUMAN]] Functions as an intracellular arginine sensor that regulates the TORC1 signaling pathway through the GATOR complex. As a homooligomer or a heterooligomer with GATSL2, directly binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of arginine to GATSL3 disrupts the interaction of GATSL3-containing oligomers with GATOR2 and activates the TORC1 signaling pathway.<ref>PMID:26972053</ref> | [[http://www.uniprot.org/uniprot/GATL3_HUMAN GATL3_HUMAN]] Functions as an intracellular arginine sensor that regulates the TORC1 signaling pathway through the GATOR complex. As a homooligomer or a heterooligomer with GATSL2, directly binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of arginine to GATSL3 disrupts the interaction of GATSL3-containing oligomers with GATOR2 and activates the TORC1 signaling pathway.<ref>PMID:26972053</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) is a master regulator of metabolism and cell growth. Among the numerous extracellular and intracellular signals, certain amino acids activate mTORC1 in a Rag-dependent manner. Arginine can stimulate mTORC1 activity by releasing the inhibitor CASTOR1 (Cellular Arginine Sensor of mTORC1) from GATOR2, a positive regulator of mTORC1 which interacts with GATOR1, the GAP for RagA/B. Three groups have resolved the structures of arginine-CASTOR1 complex, shedding a new light on molecular basis of the regulation of mTORC1 activity by arginine. However, lacking the apo structure of CASTOR1 prelimited the molecular understanding of mechanism underlying mTORC1 regulation. Here, we report crystal structures of arginine sensor CASTOR1 in arginine-bound and ligand free states at 2.05A and 2.8A, respectively. Structural comparison of CASTOR1 between two states reveals near identical conformations, except in two loop regions. It indicates CASTOR1 does not undergo large conformational change during arginine binding. Therefore, we conclude a detailed structural interpretation of arginine sensing by CASTOR1 in mTORC1 pathway. | ||
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| + | Crystal structures of arginine sensor CASTOR1 in arginine-bound and ligand free states.,Zhou Y, Wang C, Xiao Q, Guo L Biochem Biophys Res Commun. 2019 Jan 8;508(2):387-391. doi:, 10.1016/j.bbrc.2018.11.147. Epub 2018 Nov 28. PMID:30503338<ref>PMID:30503338</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5gt7" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Deng, D]] | [[Category: Deng, D]] | ||
[[Category: Guo, L]] | [[Category: Guo, L]] | ||
Revision as of 12:30, 13 March 2019
Crystal Structure of Arg-bound CASTOR1
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Categories: Human | Large Structures | Deng, D | Guo, L | Act domain | Arginine binding | Castor1 | Gator2 | Gatsl2 | Mtor | Signaling protein
