5z7x
From Proteopedia
(Difference between revisions)
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==Crystal structure of Striga hermonthica HTL4 (ShHTL4)== | ==Crystal structure of Striga hermonthica HTL4 (ShHTL4)== | ||
| - | <StructureSection load='5z7x' size='340' side='right' caption='[[5z7x]], [[Resolution|resolution]] 2.06Å' scene=''> | + | <StructureSection load='5z7x' size='340' side='right'caption='[[5z7x]], [[Resolution|resolution]] 2.06Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5z7x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z7X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5z7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buchnera_hermonthica Buchnera hermonthica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z7X FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z7x OCA], [http://pdbe.org/5z7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z7x RCSB], [http://www.ebi.ac.uk/pdbsum/5z7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z7x ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z7x OCA], [http://pdbe.org/5z7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z7x RCSB], [http://www.ebi.ac.uk/pdbsum/5z7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z7x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we show that Striga hermonthica HTL proteins (ShHTLs) in 'conserved' and 'intermediate' clades are able to bind karrikin. The 'divergent' clade is able to hydrolyze strigolactone. Unexpectedly, we find that ShD14 is also capable of hydrolyzing strigolactone. Through comparative analysis of ShHTLs and ShD14 crystal structures, we provide insights into the basis for their selectivity. Moreover, we show that both ShD14 and divergent clade ShHTLs, but not conserved and intermediate clade ShHTLs, can interact with the putative downstream signaling component ShMAX2 in the presence of the synthetic strigolactone, rac-GR24. These findings provide insight into how strigolactone is perceived and how ligand specificity is determined. | ||
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| + | Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga.,Xu Y, Miyakawa T, Nosaki S, Nakamura A, Lyu Y, Nakamura H, Ohto U, Ishida H, Shimizu T, Asami T, Tanokura M Nat Commun. 2018 Sep 26;9(1):3947. doi: 10.1038/s41467-018-06452-2. PMID:30258184<ref>PMID:30258184</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5z7x" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Buchnera hermonthica]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Miyakawa, T]] | [[Category: Miyakawa, T]] | ||
[[Category: Nakamura, A]] | [[Category: Nakamura, A]] | ||
Revision as of 12:33, 13 March 2019
Crystal structure of Striga hermonthica HTL4 (ShHTL4)
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