6a4k
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Human antibody 32D6 Fab in complex with H1N1 influenza A virus HA1== | |
| + | <StructureSection load='6a4k' size='340' side='right'caption='[[6a4k]], [[Resolution|resolution]] 3.15Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6a4k]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A4K FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4k OCA], [http://pdbe.org/6a4k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a4k RCSB], [http://www.ebi.ac.uk/pdbsum/6a4k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Influenza is a contagious acute respiratory disease caused by the influenza virus infection. Hemagglutinin (HA) is an important target in the therapeutic treatment and diagnostic detection of the influenza virus. Influenza A virus encompasses several different HA subtypes with different strains, which are constantly changing. In this study, we identified a fully human H1N1 neutralizing antibody (32D6) via an Epstein-Barr virus-immortalized B cell-based technology. 32D6 specifically neutralizes the clinically isolated H1N1 strains after the 2009 pandemic but not the earlier strains. The epitope was identified through X-ray crystallographic analysis of the 32D6-Fab/HA1 complex structure, which revealed a unique loop conformation located on the top surface of HA. The major region is composed of two peptide segments (residues 172-177 and 206-213), which form an abreast loop conformation. The residue T262 between the two loops forms a conformational epitope for recognition by 32D6. Three water molecules were observed at the interface of HA and the heavy chain, and they may constitute a stabilizing element for the 32D6-HA association. In addition, each 32D6-Fab is likely capable of blocking one HA trimer. This study provides important information on the strain specificity of 32D6 for the therapeutic treatment and detection of viral infection. | ||
| - | + | An Effective Neutralizing Antibody Against Influenza Virus H1N1 from Human B Cells.,Lee CC, Yang CY, Lin LL, Ko TP, Chang AH, Chang SS, Wang AH Sci Rep. 2019 Mar 14;9(1):4546. doi: 10.1038/s41598-019-40937-4. PMID:30872685<ref>PMID:30872685</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6a4k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ko, T P]] | ||
| + | [[Category: Lee, C C]] | ||
| + | [[Category: Lin, L L]] | ||
| + | [[Category: Wang, A H.J]] | ||
| + | [[Category: Antibody fab]] | ||
| + | [[Category: Complex structure]] | ||
| + | [[Category: Influenza hemagglutinin]] | ||
| + | [[Category: Specific binding]] | ||
| + | [[Category: Viral protein-immune system complex]] | ||
Revision as of 06:31, 27 March 2019
Human antibody 32D6 Fab in complex with H1N1 influenza A virus HA1
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