6hs7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 6hs7 is ON HOLD until Paper Publication
+
==Type VI membrane complex==
 +
<StructureSection load='6hs7' size='340' side='right'caption='[[6hs7]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[6hs7]] is a 25 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HS7 FirstGlance]. <br>
 +
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hs7 OCA], [http://pdbe.org/6hs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hs7 RCSB], [http://www.ebi.ac.uk/pdbsum/6hs7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hs7 ProSAT]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Bacteria have evolved macromolecular machineries that secrete effectors and toxins to survive and thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine that is related to Myoviridae phages. It is composed of a phage tail-like structure inserted in the bacterial cell envelope by a membrane complex (MC) comprising the TssJ, TssL and TssM proteins. We previously reported the low-resolution negative-stain electron microscopy structure of the enteroaggregative Escherichia coli MC and proposed a rotational 5-fold symmetry with a TssJ:TssL:TssM stoichiometry of 2:2:2. Here, cryo-electron tomography analyses of the T6SS MC confirm the 5-fold symmetry in situ and identify the regions of the structure that insert into the bacterial membranes. A high-resolution model obtained by single-particle cryo-electron microscopy highlights new features: five additional copies of TssJ, yielding a TssJ:TssL:TssM stoichiometry of 3:2:2, an 11-residue loop in TssM, protruding inside the lumen of the MC and constituting a functionally important periplasmic gate, and hinge regions. Based on these data, we propose an updated model on MC structure and dynamics during T6SS assembly and function.
-
Authors:
+
In situ and high-resolution cryo-EM structure of a bacterial type VI secretion system membrane complex.,Rapisarda C, Cherrak Y, Kooger R, Schmidt V, Pellarin R, Logger L, Cascales E, Pilhofer M, Durand E, Fronzes R EMBO J. 2019 Mar 15. pii: embj.2018100886. doi: 10.15252/embj.2018100886. PMID:30877094<ref>PMID:30877094</ref>
-
Description:
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
 +
<div class="pdbe-citations 6hs7" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Fronzes, R]]
 +
[[Category: Rapisarda, C]]
 +
[[Category: Membrane complex]]
 +
[[Category: Membrane protein]]
 +
[[Category: Tether]]

Revision as of 06:39, 27 March 2019

Type VI membrane complex

PDB ID 6hs7

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools