5t4a
From Proteopedia
(Difference between revisions)
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==Crystal structure of BhGH81 in complex with laminaro-hexaose== | ==Crystal structure of BhGH81 in complex with laminaro-hexaose== | ||
| - | <StructureSection load='5t4a' size='340' side='right' caption='[[5t4a]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='5t4a' size='340' side='right'caption='[[5t4a]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5t4a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T4A FirstGlance]. <br> | <table><tr><td colspan='2'>[[5t4a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T4A FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4a OCA], [http://pdbe.org/5t4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t4a RCSB], [http://www.ebi.ac.uk/pdbsum/5t4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4a ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4a OCA], [http://pdbe.org/5t4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t4a RCSB], [http://www.ebi.ac.uk/pdbsum/5t4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Family 81 glycoside hydrolases (GHs), which are known to cleave beta-1,3-glucans, are found in archaea, bacteria, eukaryotes, and viruses. Here we examine the structural and functional features of the GH81 catalytic module, BhGH81, from the Bacillus halodurans protein BH0236 to probe the molecular basis of beta-1,3-glucan recognition and cleavage. BhGH81 displayed activity on laminarin, curdlan, and pachyman, but not scleroglucan; the enzyme also cleaved beta-1,3-glucooligosaccharides as small as beta-1,3-glucotriose. The crystal structures of BhGH81 in complex with various beta-1,3-glucooligosaccharides revealed distorted sugars in the -1 catalytic subsite and an arrangement consistent with an inverting catalytic mechanism having a proposed conformational itinerary of (2)S0 --> (2,5)B(double dagger) --> (5)S1. Notably, the architecture of the catalytic site, location of an adjacent ancillary beta-1,3-glucan binding site, and the surface properties of the enzyme indicate the likely ability to recognize the double and/or triple-helical quaternary structures adopted by beta-1,3-glucans. | ||
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| + | Structural Analysis of a Family 81 Glycoside Hydrolase Implicates Its Recognition of beta-1,3-Glucan Quaternary Structure.,Pluvinage B, Fillo A, Massel P, Boraston AB Structure. 2017 Sep 5;25(9):1348-1359.e3. doi: 10.1016/j.str.2017.06.019. Epub, 2017 Aug 3. PMID:28781080<ref>PMID:28781080</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5t4a" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bachd]] | [[Category: Bachd]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Boraston, A B]] | [[Category: Boraston, A B]] | ||
[[Category: Pluvinage, B]] | [[Category: Pluvinage, B]] | ||
[[Category: Glycoside hydrolase]] | [[Category: Glycoside hydrolase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 06:54, 27 March 2019
Crystal structure of BhGH81 in complex with laminaro-hexaose
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